Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration

Cady, Emily; Orkwis, Jacob A.; Weaver, Rachel; Conlin, Lia; Madigan, Nicolas N.; Harris, Greg M.

doi:10.3390/bioengineering7030102

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Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration

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Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, OH 45221, USA

Department of Neurology, Mayo Clinic, Rochester, MN 55905, USA

Department of Biomedical Engineering, University of Cincinnati, Cincinnati, OH 45221, USA

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Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA

Author to whom correspondence should be addressed.

Bioengineering 2020, 7(3), 102; https://doi.org/10.3390/bioengineering7030102

Received: 10 July 2020 / Revised: 25 August 2020 / Accepted: 27 August 2020 / Published: 31 August 2020

(This article belongs to the Special Issue Extracellular Matrix in Wound Healing)

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Abstract

Bioactive surfaces and materials have displayed great potential in a variety of tissue engineering applications but often struggle to completely emulate complex bodily systems. The extracellular matrix (ECM) is a crucial, bioactive component in all tissues and has recently been identified as a potential solution to be utilized in combination with biomaterials. In tissue engineering, the ECM can be utilized in a variety of applications by employing the biochemical and biomechanical cues that are crucial to regenerative processes. However, viable solutions for maintaining the dimensionality, spatial orientation, and protein composition of a naturally cell-secreted ECM remain challenging in tissue engineering. Therefore, this work used soft lithography to create micropatterned polydimethylsiloxane (PDMS) substrates of a three-dimensional nature to control cell adhesion and alignment. Cells aligned on the micropatterned PDMS, secreted and assembled an ECM, and were decellularized to produce an aligned matrix biomaterial. The cells seeded onto the decellularized, patterned ECM showed a high degree of alignment and migration along the patterns compared to controls. This work begins to lay the groundwork for elucidating the immense potential of a natural, cell-secreted ECM for directing cell function and offers further guidance for the incorporation of natural, bioactive components for emerging tissue engineering technologies. View Full-Text

Keywords: extracellular matrix; decellularization; micropatterning; cell alignment; biomaterials; tissue engineering

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MDPI and ACS Style

Cady, E.; Orkwis, J.A.; Weaver, R.; Conlin, L.; Madigan, N.N.; Harris, G.M. Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration. Bioengineering 2020, 7, 102. https://doi.org/10.3390/bioengineering7030102

AMA Style

Cady E, Orkwis JA, Weaver R, Conlin L, Madigan NN, Harris GM. Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration. Bioengineering. 2020; 7(3):102. https://doi.org/10.3390/bioengineering7030102

Chicago/Turabian Style

Cady, Emily, Jacob A. Orkwis, Rachel Weaver, Lia Conlin, Nicolas N. Madigan, and Greg M. Harris. 2020. "Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration" Bioengineering 7, no. 3: 102. https://doi.org/10.3390/bioengineering7030102

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Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration

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