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Expansion Culture of Human Pluripotent Stem Cells and Production of Cardiomyocytes

Institute for Integrated Cell-Material Sciences (iCeMS), Institute for Advanced Study, Kyoto University, Kyoto 606-8501, Japan
Author to whom correspondence should be addressed.
Bioengineering 2019, 6(2), 48;
Received: 13 March 2019 / Revised: 15 May 2019 / Accepted: 18 May 2019 / Published: 24 May 2019
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture, Volume 2)
PDF [1820 KB, uploaded 24 May 2019]


Transplantation of human pluripotent stem cell (hPSCs)-derived cardiomyocytes for the treatment of heart failure is a promising therapy. In order to implement this therapy requiring numerous cardiomyocytes, substantial production of hPSCs followed by cardiac differentiation seems practical. Conventional methods of culturing hPSCs involve using a 2D culture monolayer that hinders the expansion of hPSCs, thereby limiting their productivity. Advanced culture of hPSCs in 3D aggregates in the suspension overcomes the limitations of 2D culture and attracts immense attention. Although the hPSC production needs to be suitable for subsequent cardiac differentiation, many studies have independently focused on either expansion of hPSCs or cardiac differentiation protocols. In this review, we summarize the recent approaches to expand hPSCs in combination with cardiomyocyte differentiation. A comparison of various suspension culture methods and future prospects for dynamic culture of hPSCs are discussed in this study. Understanding hPSC characteristics in different models of dynamic culture helps to produce numerous cells that are useful for further clinical applications. View Full-Text
Keywords: human pluripotent stem cells (hPSCs); expansion culture; cardiomyocyte differentiation human pluripotent stem cells (hPSCs); expansion culture; cardiomyocyte differentiation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Le, M.N.T.; Hasegawa, K. Expansion Culture of Human Pluripotent Stem Cells and Production of Cardiomyocytes. Bioengineering 2019, 6, 48.

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