DNA-Based Tools to Certify Authenticity of Rice Varieties—An Overview
Round 1
Reviewer 1 Report
I found the review very interesting and up to day. The rice is a crop with high food market interest all over the world, not only in Europe. The DNA-based tools to certify authenticity of rice varieties are key methods to keep the market clean and safe for buyers and consumers. It is also important for scientists focusing on rice research and experiments. The paper presents basic knowledge on DNA based methods for rice authentication based on newest scientific literature. The main drawback of the paper is lack of Figures an Tables that will help readers to understand and follow the text. I advise to supplement the text with figures for rice production/consumption as well as in case of separate analytical methods presented in the text. Additionally in my opinion the Figure 1 is rather useless. I see no reason to present such a data. I believe paper also needs some language polish done by some English native speaker. It will help readers to follow the main idea of the paper as well as makes paper more readable.
According to this I have to designate the paper as needed minor revision.
Author Response
"Please see the attachment."
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Well-written manuscript. Some comments:
Line 12: Mediterranean
Line 37: a long time
Line 53: originally from West Africa
Line 76: constraints
Lines 88-95: Put references
Line 127: pandemic
Lines 147-148: There are no Sections 4 and 5. Only Sections 2 and 3 ? Please kindly check.
Line 280: amplified locus
Lines 294-295: annotation, annotated
Line 301: essential
Line 318: Include references for these two articles.
Line 329: eight
Line 334: scaling-up
Line 351: cases. (remove the second period)
Line 361: Chloroplast genome super DNA barcodes use
Line 366: advancement
Line 371: dimension
Line 408: unravel
Line 413: Authentication
Line 433: Linear After Exponential
Line 444: when raising the temperature
Line 454: in opposition
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
Reviewer 3 Report
1.From the perspective of food safety, is the mixture of new rice and old rice also a kind of food adulteration? Kit can detect the variety, but cannot detect the new and old. Moreover, the old rice will have food safety problems if the quality deteriorates under long-term storage?
2.DNA-based tools to certify authenticity of rice varieties. When the varieties are from the same source, but the place of origin is different, can DNA-based tools can be used to control the quality of rice?
3.From the perspective of food safety, is the mixture of new rice and old rice also a kind of food adulteration? Kit can detect the variety, but cannot detect the new and old. Moreover, the old rice will have food safety problems if the quality deteriorates under long-term storage?
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
