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Dentistry Journal
Volume 13
Issue 10
10.3390/dj13100460
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

From Bench to Chairside: Collagen Scaffolds in Combination with Mesenchymal Stromal Cells for Gingival Augmentation

by
Polina Koteneva
1,
Nastasia Kosheleva
1,
Alexey Fayzullin
1,
Yana Khristidis
1,
Egor Bunin
1,
Yuri Efremov
1,
Diana Zhukova
1,
Sergey Tkachev
1,
Aida Kulova
2,
Timur Rasulov
2,
Anna Vedyaeva
2,3,
Tatiana Brailovskaya
2,3 and
Peter Timashev
1,*
1
Institute for Regenerative Medicine, Sechenov University, 119991 Moscow, Russia
2
Central Research Institute of Dentistry and Maxillofacial Surgery, 119991 Moscow, Russia
3
E.V. Borovsky Institute of Dentistry, Sechenov University, 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
Dent. J. 2025, 13(10), 460; https://doi.org/10.3390/dj13100460 (registering DOI)
Submission received: 24 July 2025 / Revised: 11 September 2025 / Accepted: 29 September 2025 / Published: 8 October 2025
(This article belongs to the Special Issue Regenerative Approaches in Dental Sciences)
Versions Notes

Abstract

Background/Objectives: Gingival tissue deficiencies present significant treatment challenges. We investigated three xenogeneic collagen scaffolds—Fibro-Gide, FibroMATRIX, and Mucoderm—with and without human gingival MSCs for soft tissue augmentation. Methods: The study assessed scaffold properties (mechanical properties and micro-CT structure), cytocompatibility, ex vivo vascular growth stimulation (CAM-test), and in vivo effects in rabbit model. Results: All scaffolds were cytocompatible and maintained MSC viability via extract and contact cytotoxicity tests. Fibro-Gide showed the highest porosity at 78.5%, followed by FibroMATRIX at 64.3%, while Mucoderm had the lowest porosity at 33.2%. Mucoderm exhibited the greatest stiffness due to its dense structure, contrasting with the more similar mechanical properties of Fibro-Gide and FibroMATRIX. In an ex vivo HET-Cam model of the angiogenic response, Fibro-Gide exhibited reduced blood vessel length and blood flow rate compared to FibroMATRIX and Mucoderm. In vivo, Mucoderm resorbed completely, FibroMATRIX demonstrated optimal partial degradation, and Fibro-Gide retained most of its collagen structure. Conclusions: The FibroMATRIX with MSCs combination showed particularly promising results for enhancing tissue thickness and vascularization, suggesting this approach could significantly improve gingival regeneration outcomes.
Keywords: gingiva soft tissue augmentation; angiogenesis; vascularization; MSC; mesenchymal stromal cells; xenogenic materials; collagen scaffolds; Mucoderm; Fibro-Gide; FibroMATRIX; dentistry; regenerative medicine gingiva soft tissue augmentation; angiogenesis; vascularization; MSC; mesenchymal stromal cells; xenogenic materials; collagen scaffolds; Mucoderm; Fibro-Gide; FibroMATRIX; dentistry; regenerative medicine

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MDPI and ACS Style

Koteneva, P.; Kosheleva, N.; Fayzullin, A.; Khristidis, Y.; Bunin, E.; Efremov, Y.; Zhukova, D.; Tkachev, S.; Kulova, A.; Rasulov, T.; et al. From Bench to Chairside: Collagen Scaffolds in Combination with Mesenchymal Stromal Cells for Gingival Augmentation. Dent. J. 2025, 13, 460. https://doi.org/10.3390/dj13100460

AMA Style

Koteneva P, Kosheleva N, Fayzullin A, Khristidis Y, Bunin E, Efremov Y, Zhukova D, Tkachev S, Kulova A, Rasulov T, et al. From Bench to Chairside: Collagen Scaffolds in Combination with Mesenchymal Stromal Cells for Gingival Augmentation. Dentistry Journal. 2025; 13(10):460. https://doi.org/10.3390/dj13100460

Chicago/Turabian Style

Koteneva, Polina, Nastasia Kosheleva, Alexey Fayzullin, Yana Khristidis, Egor Bunin, Yuri Efremov, Diana Zhukova, Sergey Tkachev, Aida Kulova, Timur Rasulov, and et al. 2025. "From Bench to Chairside: Collagen Scaffolds in Combination with Mesenchymal Stromal Cells for Gingival Augmentation" Dentistry Journal 13, no. 10: 460. https://doi.org/10.3390/dj13100460

APA Style

Koteneva, P., Kosheleva, N., Fayzullin, A., Khristidis, Y., Bunin, E., Efremov, Y., Zhukova, D., Tkachev, S., Kulova, A., Rasulov, T., Vedyaeva, A., Brailovskaya, T., & Timashev, P. (2025). From Bench to Chairside: Collagen Scaffolds in Combination with Mesenchymal Stromal Cells for Gingival Augmentation. Dentistry Journal, 13(10), 460. https://doi.org/10.3390/dj13100460

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