The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Skeletal Muscle Cell-Line Demographics
2.2. Media Preparation
2.3. Skeletal Muscle Cell Maintenance
2.4. Sub-Culturing the Culture Vessel
2.5. Skeletal Muscle Cell Count and Viability
2.6. Thermotherapy Treatments
2.7. RNA Purification
2.8. Gene Expression
2.9. Cytokine Inflammatory Markers
2.10. Statistical Analysis
3. Results
3.1. Skeletal Muscle Cell Viability
3.2. Gene Expression
3.3. Cytokine Inflammatory Markers
3.3.1. Interleukin-1β
3.3.2. Interleukin-4
3.3.3. Interleukin-6
3.3.4. Interleukin-10
3.3.5. Tumor Necrosis Factor-α
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Bachnak, L.; Sparks, J.; Newmire, D.E.; Gonzales, X.F.; Omoruyi, F.O. The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers. Biomedicines 2021, 9, 1276. https://doi.org/10.3390/biomedicines9091276
Bachnak L, Sparks J, Newmire DE, Gonzales XF, Omoruyi FO. The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers. Biomedicines. 2021; 9(9):1276. https://doi.org/10.3390/biomedicines9091276
Chicago/Turabian StyleBachnak, Louay, Jean Sparks, Daniel E. Newmire, Xavier F. Gonzales, and Felix O. Omoruyi. 2021. "The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers" Biomedicines 9, no. 9: 1276. https://doi.org/10.3390/biomedicines9091276