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Article

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

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CEITEC–Central European Institute of Technology, Brno University of Technology, 612 00 Brno, Czech Republic
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Faculty of Medicine, Department of Burns and Plastic Surgery, Institution Shared with the University Hospital Brno, 625 00 Brno, Czech Republic
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Clinic of Plastic and Esthetic Surgery, St Anne’s University Hospital, 602 00 Brno, Czech Republic
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Veterinary Research Institute, 621 00 Brno, Czech Republic
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Faculty of Medicine, Institute of Pathology, University Hospital Brno, Masaryk University, 625 00 Brno, Czech Republic
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Institute of Experimental Medicine of the Czech Academy of Science, 142 20 Prague, Czech Republic
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Department of Materials Science and Engineering, Kroto Research Institute, North Campus, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Bilal Al-Nawas and Peer Wolfgang Kämmerer
Biomedicines 2021, 9(6), 590; https://doi.org/10.3390/biomedicines9060590
Received: 19 April 2021 / Revised: 13 May 2021 / Accepted: 19 May 2021 / Published: 22 May 2021
Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 °C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB® were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB® already at very low concentrations (0.01 µg/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB® protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration. View Full-Text
Keywords: collagen; chitosan; scaffold; FGF2; skin regeneration; tissue engineering collagen; chitosan; scaffold; FGF2; skin regeneration; tissue engineering
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MDPI and ACS Style

Vojtová, L.; Pavliňáková, V.; Muchová, J.; Kacvinská, K.; Brtníková, J.; Knoz, M.; Lipový, B.; Faldyna, M.; Göpfert, E.; Holoubek, J.; Pavlovský, Z.; Vícenová, M.; Blahnová, V.H.; Hearnden, V.; Filová, E. Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation. Biomedicines 2021, 9, 590. https://doi.org/10.3390/biomedicines9060590

AMA Style

Vojtová L, Pavliňáková V, Muchová J, Kacvinská K, Brtníková J, Knoz M, Lipový B, Faldyna M, Göpfert E, Holoubek J, Pavlovský Z, Vícenová M, Blahnová VH, Hearnden V, Filová E. Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation. Biomedicines. 2021; 9(6):590. https://doi.org/10.3390/biomedicines9060590

Chicago/Turabian Style

Vojtová, Lucy, Veronika Pavliňáková, Johana Muchová, Katarína Kacvinská, Jana Brtníková, Martin Knoz, Břetislav Lipový, Martin Faldyna, Eduard Göpfert, Jakub Holoubek, Zdeněk Pavlovský, Monika Vícenová, Veronika Hefka Blahnová, Vanessa Hearnden, and Eva Filová. 2021. "Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation" Biomedicines 9, no. 6: 590. https://doi.org/10.3390/biomedicines9060590

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