Next Article in Journal
Interstitial Pneumonia with Autoimmune Features: Why Rheumatologist-Pulmonologist Collaboration Is Essential
Next Article in Special Issue
Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix
Previous Article in Journal
Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells
Previous Article in Special Issue
Angiogenic Potential in Biological Hydrogels
Open AccessArticle

Light Cross-Linkable Marine Collagen for Coaxial Printing of a 3D Model of Neuromuscular Junction Formation

ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Wollongong, New South Wales 2500, Australia
*
Author to whom correspondence should be addressed.
Current address: Department of Orthopedics, University Medical Center Utrecht, Utrecht 3508 GA, The Netherlands.
Biomedicines 2021, 9(1), 16; https://doi.org/10.3390/biomedicines9010016
Received: 15 December 2020 / Revised: 21 December 2020 / Accepted: 22 December 2020 / Published: 26 December 2020
(This article belongs to the Special Issue Hydrogels for Biomedical Application)
Collagen is a major component of the extracellular matrix (ECM) that modulates cell adhesion, growth, and migration, and has been utilised in tissue engineering applications. However, the common terrestrial sources of collagen carry the risk of zoonotic disease transmission and there are religious barriers to the use of bovine and porcine products in many cultures. Marine based collagens offer an attractive alternative and have so far been under-utilized for use as biomaterials for tissue engineering. Marine collagen can be extracted from fish waste products, therefore industry by-products offer an economical and environmentally sustainable source of collagen. In a handful of studies, marine collagen has successfully been methacrylated to form collagen methacrylate (ColMA). Our work included the extraction, characterization and methacrylation of Red Snapper collagen, optimisation of conditions for neural cell seeding and encapsulation using the unmodified collagen, thermally cross-linked, and the methacrylated collagen with UV-induced cross-linking. Finally, the 3D co-axial printing of neural and skeletal muscle cell cultures as a model for neuromuscular junction (NMJ) formation was investigated. Overall, the results of this study show great potential for a novel NMJ in vitro 3D bioprinted model that, with further development, could provide a low-cost, customizable, scalable and quick-to-print platform for drug screening and to study neuromuscular junction physiology and pathogenesis. View Full-Text
Keywords: 3D bioprinting; neural cell; skeletal muscle cell; neuromuscular junction 3D bioprinting; neural cell; skeletal muscle cell; neuromuscular junction
Show Figures

Figure 1

MDPI and ACS Style

Sanz, B.; Albillos Sanchez, A.; Tangey, B.; Gilmore, K.; Yue, Z.; Liu, X.; Wallace, G. Light Cross-Linkable Marine Collagen for Coaxial Printing of a 3D Model of Neuromuscular Junction Formation. Biomedicines 2021, 9, 16. https://doi.org/10.3390/biomedicines9010016

AMA Style

Sanz B, Albillos Sanchez A, Tangey B, Gilmore K, Yue Z, Liu X, Wallace G. Light Cross-Linkable Marine Collagen for Coaxial Printing of a 3D Model of Neuromuscular Junction Formation. Biomedicines. 2021; 9(1):16. https://doi.org/10.3390/biomedicines9010016

Chicago/Turabian Style

Sanz, Borja; Albillos Sanchez, Ane; Tangey, Bonnie; Gilmore, Kerry; Yue, Zhilian; Liu, Xiao; Wallace, Gordon. 2021. "Light Cross-Linkable Marine Collagen for Coaxial Printing of a 3D Model of Neuromuscular Junction Formation" Biomedicines 9, no. 1: 16. https://doi.org/10.3390/biomedicines9010016

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop