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Identification of Differentially Methylated CpG Sites in Fibroblasts from Keloid Scars
Article

Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

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Department of Anatomy, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia
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Genomics and Personalized Medicine Unit, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia
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Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan
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Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid 22110, Jordan
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Burn Injury Research Unit, School of Biomedical Sciences, Faculty of Health and Medical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia
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School of Medicine, The University of Notre Dame Australia, Fremantle 6959, Australia
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Department of Dermatology and Copenhagen Wound Healing Center, Copenhagen University Hospital, DK-2400 Copenhagen NV, Denmark
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Telethon Kids Institute, Perth Children’s Hospital, The University of Western Australia, Nedlands 6009, Australia
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Burns Service of Western Australia, Perth Children’s Hospital and Fiona Stanley Hospital, Department of Health, Perth 6009, Australia
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Fiona Wood Foundation, Fiona Stanley Hospital, Murdoch, Perth 6150, Australia
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Author to whom correspondence should be addressed.
Biomedicines 2020, 8(7), 200; https://doi.org/10.3390/biomedicines8070200
Received: 6 June 2020 / Revised: 1 July 2020 / Accepted: 2 July 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Cellular Mechanisms in Wound Healing)
Interactions between keratinocytes and fibroblasts in the skin layers are crucial in normal tissue development, wound healing, and scarring. This study has investigated the role of keloid keratinocytes in regulating collagen production by primary fibroblasts in vitro. Keloid cells were obtained from removed patients’ tissue whereas normal skin cells were discarded tissue obtained from elective surgery procedures. Fibroblasts and keratinocytes were isolated, cultured, and a transwell co-culture system were used to investigate the effect of keratinocytes on collagen production using a ‘scar-in-a-jar’ model. Keloid fibroblasts produced significantly more collagen than normal skin fibroblasts in monoculture at the RNA, secreted protein, and stable fibrillar protein level. When keloid keratinocytes were added to normal skin fibroblasts, expression of collagen was significantly upregulated in most samples, but when added to keloid fibroblasts, collagen I production was significantly reduced. Interestingly, keloid keratinocytes appear to decrease collagen production by keloid fibroblasts. This suggests that signaling in both keratinocytes and fibroblasts is disrupted in keloid pathology. View Full-Text
Keywords: keloids; keratinocytes; collagen type I; wound healing; fibroblasts; coculture techniques keloids; keratinocytes; collagen type I; wound healing; fibroblasts; coculture techniques
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MDPI and ACS Style

Alghamdi, M.A.; AL-Eitan, L.N.; Stevenson, A.; Chaudhari, N.; Hortin, N.; Wallace, H.J.; Danielsen, P.L.; Manzur, M.; Wood, F.M.; Fear, M.W. Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study. Biomedicines 2020, 8, 200. https://doi.org/10.3390/biomedicines8070200

AMA Style

Alghamdi MA, AL-Eitan LN, Stevenson A, Chaudhari N, Hortin N, Wallace HJ, Danielsen PL, Manzur M, Wood FM, Fear MW. Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study. Biomedicines. 2020; 8(7):200. https://doi.org/10.3390/biomedicines8070200

Chicago/Turabian Style

Alghamdi, Mansour A., Laith N. AL-Eitan, Andrew Stevenson, Nutan Chaudhari, Nicole Hortin, Hilary J. Wallace, Patricia L. Danielsen, Mitali Manzur, Fiona M. Wood, and Mark W. Fear 2020. "Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study" Biomedicines 8, no. 7: 200. https://doi.org/10.3390/biomedicines8070200

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