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Open AccessArticle

A Protocol for the Multi-Omic Integration of Cervical Microbiota and Urine Metabolomics to Understand Human Papillomavirus (HPV)-Driven Dysbiosis

1
Department of Biochemistry, UPR School of Medicine, 00921 San Juan, Puerto Rico
2
Department of Microbiology & Medical Zoology, UPR School of Medicine, 00921 San Juan, Puerto Rico
*
Author to whom correspondence should be addressed.
Biomedicines 2020, 8(4), 81; https://doi.org/10.3390/biomedicines8040081
Received: 21 February 2020 / Revised: 3 April 2020 / Accepted: 6 April 2020 / Published: 8 April 2020
(This article belongs to the Section Cancer Therapeutics)
The multi-omic integration of microbiota data with metabolomics has gained popularity. This protocol is based on a human multi-omics study, integrating cervicovaginal microbiota, HPV status and neoplasia, with urinary metabolites. Indeed, to understand the biology of the infections and to develop adequate interventions for cervical cancer prevention, studies are needed to characterize in detail the cervical microbiota and understand the systemic metabolome. This article is a detailed protocol for the multi-omic integration of cervical microbiota and urine metabolome to shed light on the systemic effects of cervical dysbioses associated with Human Papillomavirus (HPV) infections. This methods article suggests detailed sample collection and laboratory processes of metabolomics, DNA extraction for microbiota, HPV typing, and the bioinformatic analyses of the data, both to characterize the metabolome, the microbiota, and joint multi-omic analyses, useful for the development of new point-of-care diagnostic tests based on these approaches. View Full-Text
Keywords: multi-omics; cervical epithelial microenvironment; HPV; 16S rRNA; metabolomics multi-omics; cervical epithelial microenvironment; HPV; 16S rRNA; metabolomics
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Chorna, N.; Godoy-Vitorino, F. A Protocol for the Multi-Omic Integration of Cervical Microbiota and Urine Metabolomics to Understand Human Papillomavirus (HPV)-Driven Dysbiosis. Biomedicines 2020, 8, 81.

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