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Article

A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO2 Laser Drilling

1
Department of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan
2
Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan
3
Medical Device Innovation Center, National Cheng Kung University, Tainan 70101, Taiwan
4
International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan 70101, Taiwan
*
Author to whom correspondence should be addressed.
Biomedicines 2020, 8(10), 427; https://doi.org/10.3390/biomedicines8100427
Received: 26 September 2020 / Accepted: 15 October 2020 / Published: 18 October 2020
(This article belongs to the Section Biomedical Materials and Nanomedicine)
CO2 laser manufacturing has served as an enabling and reliable tool for rapid and cost-effective microfabrication over the past few decades. While a wide range of industrial and biological applications have been studied, the choice of materials fabricated across various laser parameters and systems is often confounded by their complex combinations. We herein presented a unified procedure performed using percussion CO2 laser drilling with a range of laser parameters, substrate materials and various generated microstructures, enabling a variety of downstream tissue/cellular-based applications. Emphasis is placed on delineating the laser drilling effect on different biocompatible materials and proof-of-concept utilities. First, a polydimethylsiloxane (PDMS) microneedle (MN) array mold is fabricated to generate dissolvable polyvinylpyrrolidone/polyvinyl alcohol (PVP/PVA) MNs for transdermal drug delivery. Second, polystyrene (PS) microwells are optimized in a compact array for the formation of size-controlled multicellular tumor spheroids (MCTSs). Third, coverglass is perforated to form a microaperture that can be used to trap/position cells/spheroids. Fourth, the creation of through-holes in PS is validated as an accessible method to create channels that facilitate medium exchange in hanging drop arrays and as a conducive tool for the growth and drug screenings of MCTSs. View Full-Text
Keywords: CO2 laser; rapid prototyping; microneedle; multicellular tumor spheroids; microwells; hanging drops CO2 laser; rapid prototyping; microneedle; multicellular tumor spheroids; microwells; hanging drops
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MDPI and ACS Style

Chen, Y.-W.; Chen, M.-C.; Wu, K.-W.; Tu, T.-Y. A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO2 Laser Drilling. Biomedicines 2020, 8, 427. https://doi.org/10.3390/biomedicines8100427

AMA Style

Chen Y-W, Chen M-C, Wu K-W, Tu T-Y. A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO2 Laser Drilling. Biomedicines. 2020; 8(10):427. https://doi.org/10.3390/biomedicines8100427

Chicago/Turabian Style

Chen, Yu-Wei, Mei-Chin Chen, Kuang-Wei Wu, and Ting-Yuan Tu. 2020. "A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO2 Laser Drilling" Biomedicines 8, no. 10: 427. https://doi.org/10.3390/biomedicines8100427

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