Next Article in Journal
Local Factors Rather than the Landscape Context Explain Species Richness and Functional Trait Diversity and Responses of Plant Assemblages of Mediterranean Cereal Field Margins
Previous Article in Journal
Nitric Oxide Pre-Treatment Advances Seed Germination and Alleviates Copper-Induced Photosynthetic Inhibition in Indian Mustard
Open AccessArticle

The Presence of Plant-Associated Bacteria Alters Responses to N-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in Medicago Truncatula

1
Division of Plant Sciences, Research School of Biology, Canberra, ACT 2601, Australia
2
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
*
Author to whom correspondence should be addressed.
Plants 2020, 9(6), 777; https://doi.org/10.3390/plants9060777
Received: 25 May 2020 / Revised: 14 June 2020 / Accepted: 15 June 2020 / Published: 22 June 2020
(This article belongs to the Section Plant–Soil Interactions)
Bacteria use quorum sensing signaling for cell-to-cell communication, which is also important for their interactions with plant hosts. Quorum sensing via N-acyl-homoserine lactones (AHLs) is important for successful symbioses between legumes and nitrogen-fixing rhizobia. Previous studies have shown that plant hosts can recognize and respond to AHLs. Here, we tested whether the response of the model legume Medicago truncatula to AHLs from its symbiont and other bacteria could be modulated by the abundance and composition of plant-associated microbial communities. Temporary antibiotic treatment of the seeds removed the majority of bacterial taxa associated with M. truncatula roots and significantly altered the effect of AHLs on nodule numbers, but lateral root density, biomass, and root length responses were much less affected. The AHL 3-oxo-C14-HSL (homoserine lactone) specifically increased nodule numbers but only after the treatment of seeds with antibiotics. This increase was associated with increased expression of the early nodulation genes RIP1 and ENOD11 at 24 h after infection. A 454 pyrosequencing analysis of the plant-associated bacteria showed that antibiotic treatment had the biggest effect on bacterial community composition. However, we also found distinct effects of 3-oxo-C14-HSL on the abundance of specific bacterial taxa. Our results revealed a complex interaction between plants and their associated microbiome that could modify plant responses to AHLs. View Full-Text
Keywords: acyl-homoserine lactones; legume; microbiome; nodulation; rhizobia; quorum sensing acyl-homoserine lactones; legume; microbiome; nodulation; rhizobia; quorum sensing
Show Figures

Graphical abstract

MDPI and ACS Style

Veliz-Vallejos, D.F.; Kawasaki, A.; Mathesius, U. The Presence of Plant-Associated Bacteria Alters Responses to N-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in Medicago Truncatula. Plants 2020, 9, 777.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop