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Article

Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber

by 1,2,†, 1,3,†, 2, 2, 1, 1, 2 and 1,*
1
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
2
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
3
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2020, 9(3), 400; https://doi.org/10.3390/plants9030400
Received: 6 January 2020 / Revised: 1 February 2020 / Accepted: 2 March 2020 / Published: 24 March 2020
(This article belongs to the Special Issue Molecular Breeding in Horticultural Plants)
Stress-associated proteins (SAPs) are a class of zinc finger proteins that confer tolerance to a variety of abiotic and biotic stresses in diverse plant species. However, in cucumber (Cucumis sativus L.), very little is known about the roles of SAP gene family members in regulating plant growth, development, and stress responses. In this study, a total of 12 SAP genes (named as CsSAP1-CsSAP12) were identified in the cucumber genome, which were unevenly distributed on six chromosomes. Gene duplication analysis detected one tandem duplication and two segmental duplication events. Phylogenetic analysis of SAP proteins from cucumber and other plants suggested that they could be divided into seven groups (sub-families), and proteins in the same group generally had the same arrangement of AN1 (ZnF-AN1) and A20 (ZnF-A20) domains. Most of the CsSAP genes were intronless and harbored a number of stress- and hormone-responsive cis-elements in their promoter regions. Tissue expression analysis showed that the CsSAP genes had a broad spectrum of expression in different tissues, and some of them displayed remarkable alteration in expression during fruit development. RT-qPCR results indicated that all the selected CsSAP genes displayed transcriptional responses to cold, drought, and salt stresses. These results enable the first comprehensive description of the SAP gene family in cucumber and lay a solid foundation for future research on the biological functions of CsSAP genes. View Full-Text
Keywords: cucumber; stress-associated protein (SAP); abiotic stress; expression profile; gene family cucumber; stress-associated protein (SAP); abiotic stress; expression profile; gene family
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MDPI and ACS Style

Lai, W.; Zhou, Y.; Pan, R.; Liao, L.; He, J.; Liu, H.; Yang, Y.; Liu, S. Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber. Plants 2020, 9, 400. https://doi.org/10.3390/plants9030400

AMA Style

Lai W, Zhou Y, Pan R, Liao L, He J, Liu H, Yang Y, Liu S. Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber. Plants. 2020; 9(3):400. https://doi.org/10.3390/plants9030400

Chicago/Turabian Style

Lai, Wei, Yong Zhou, Rao Pan, Liting Liao, Juncheng He, Haoju Liu, Yingui Yang, and Shiqiang Liu. 2020. "Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber" Plants 9, no. 3: 400. https://doi.org/10.3390/plants9030400

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