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The Dynamics of Cytokinin Changes after Grafting of Vegetative Apices on Flowering Rapeseed Plants

1
Laboratory of Growth Regulators, The Czech Academy of Sciences, Institute of Experimental Botany & Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czechia
2
Department of Biochemistry, Biophysics and Biotechnology, Pedagogical University, Podchorązych 2, 30-084 Krakow, Poland
3
Institute of Experimental Botany, The Czech Academy of Sciences, Rozvojová 263, CZ-165 02 Prague 6—Lysolaje, Czechia
4
Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland
5
Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland
*
Author to whom correspondence should be addressed.
Plants 2019, 8(4), 78; https://doi.org/10.3390/plants8040078
Received: 20 February 2019 / Revised: 25 March 2019 / Accepted: 26 March 2019 / Published: 28 March 2019
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Abstract

Despite numerous studies, the role of hormones in the induction of shoot apical meristem leading to reproductive development, especially regarding thermoperiodic plants, is still not fully understood. The key problem is separating the effects of the low temperature required for vernalization from those responsible for low temperature stress. An earlier experiment demonstrated the correlation between an increase of cytokinin level in the apical parts of winter rapeseed and the transition time into their reproductive phase during vernalization, i.e., low temperature treatment. From data obtained from the presented experiments, this study aims to contribute to the understanding the role of cytokinins in the induction of flowering based on the grafting of vegetative apical parts of winter rapeseed (scion) on the reproductive (stock) winter and spring genotypes. On the basis of analyses carried out using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry in combination with microscopic observation of changes at the apical meristem, it was indicated that the increase in the amount of trans-zeatin and trans- and cis-zeatin-O-glucoside derivatives appeared in the early stages of apex floral differentiation. During further development, the content of all investigated cytokinins passed through the maximum level followed by their decrease. The final level in reproductive apices was found to be higher than that in vegetative ones. View Full-Text
Keywords: cytokinins; reproductive development; Brassica napus; grafting; shoot apical meristem cytokinins; reproductive development; Brassica napus; grafting; shoot apical meristem
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Tarkowská, D.; Filek, M.; Krekule, J.; Biesaga-Kościelniak, J.; Marciñska, I.; Popielarska-Konieczna, M.; Strnad, M. The Dynamics of Cytokinin Changes after Grafting of Vegetative Apices on Flowering Rapeseed Plants. Plants 2019, 8, 78.

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