MeNINV1: An Alkaline/Neutral Invertase Gene of Manihot esculenta, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Functional Analysis of MeNINV1 by Yeast Complementation
2.2. Analysis of MeNINV1 Enzymatic Characteristics
2.3. Subcellular Localization of the MeNINV1 Protein
2.4. The Expression of MeNINV1 in Cassava Tissues and during Tuber Root Development
2.5. Transformation and Identification of MeNINV1 Transgenic Arabidopsis
2.6. Overexpression of MeNINV1 in Arabidopsis Promoted Plant Growth and Delayed Flowering Time
2.7. Overexpression of MeNINV1 in Arabidopsis Increased Sucrose Metabolism and Starch Synthesis
2.8. No Change in the Overexpression of MeNINV1 in Arabidopsis under Abiotic Stress
3. Discussion
4. Materials and Methods
4.1. Strains and Plasmids
4.2. Functional Analysis of MeNINV1 by Yeast Complementation
4.3. Analysis of the Enzymatic Characteristics of MeNINV1
4.4. Plant Materials
4.5. Expression Analysis of the MeNINV1 Gene in Cassava
4.6. Subcellular Localization of the MeNINV1 Protein
4.7. Transformation and Identification of MeNINV1 Arabidopsis
4.8. Phenotypic Statistics of Transgenic Arabidopsis
4.9. Detection of Various Sugars in Transgenic Arabidopsis
4.10. Analysis of Abiotic Stress in Transgenic Arabidopsis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Chemicals | Concentration (mM) | Relative Activity (%) | Metal Ions | Concentration (mM) | Relative Activity (%) |
---|---|---|---|---|---|
None | - | 100 ± 6.54 | Ca2+ | 20 | 81.22 ± 0.47 |
Tris | 1 | 90.32 ± 7.84 | 40 | 33.25 ± 0.55 | |
2 | 84.85 ± 4.15 | 60 | 22.29 ± 0.57 | ||
4 | 75.53 ± 3.23 | 80 | 43.85 ± 0.12 | ||
6 | 69.33 ± 3.52 | 100 | 41.29 ± 0.46 | ||
8 | 62.44 ± 1.97 | Mg2+ | 20 | 67.54 ± 0.32 | |
10 | 56.15 ± 2.72 | 40 | 52.39 ± 1.66 | ||
20 | 41.72 ± 2.32 | 60 | 40.87 ± 0.19 | ||
50 | 30.70 ± 0.56 | 80 | 32.50 ± 0.63 | ||
100 | 30.11 ± 0.13 | 100 | 24.18 ± 0.56 | ||
EDTA | 20 | 52.28 ± 0.28 | Mn2+ | 20 | 46.63 ± 0.14 |
40 | 46.06 ± 0.38 | 40 | 18.86 ± 0.28 | ||
60 | 31.20 ± 0.41 | 60 | 27.91 ± 0.48 | ||
80 | 19.18 ± 0.13 | 80 | 31.54 ± 0.36 | ||
100 | 15.46 ± 0.16 | 100 | 38.93 ± 0.17 | ||
DTT | 20 | 80.71 ± 1.99 | Zn2+ | 20 | 44.60 ± 0.14 |
40 | 68.43 ± 0.21 | 40 | 15.77 ± 0.25 | ||
60 | 33.80 ± 0.10 | 60 | 12.97 ± 0.05 | ||
80 | 25.17 ± 0.21 | 80 | 12.95 ± 0.04 | ||
100 | 25.34 ± 0.44 | 100 | 12.83 ± 0.14 | ||
VB6 | 20 | 24.83 ± 0.13 | Pb2+ | 20 | 48.64 ± 0.11 |
40 | 15.20 ± 0.09 | 40 | 13.28 ± 0.02 | ||
60 | 14.17 ± 0.08 | 60 | 13.28 ± 0.13 | ||
80 | 15.97 ± 0.11 | 80 | 13.63 ± 0.09 | ||
100 | 16.59 ± 0.30 | 100 | 13.36 ± 0.06 |
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Wang, Y.-J.; Zhen, X.-H.; Zhou, Y.-J.; Wang, Y.-L.; Hou, J.-Y.; Wang, X.; Li, R.-M.; Liu, J.; Hu, X.-W.; Geng, M.-T.; et al. MeNINV1: An Alkaline/Neutral Invertase Gene of Manihot esculenta, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic Arabidopsis. Plants 2022, 11, 946. https://doi.org/10.3390/plants11070946
Wang Y-J, Zhen X-H, Zhou Y-J, Wang Y-L, Hou J-Y, Wang X, Li R-M, Liu J, Hu X-W, Geng M-T, et al. MeNINV1: An Alkaline/Neutral Invertase Gene of Manihot esculenta, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic Arabidopsis. Plants. 2022; 11(7):946. https://doi.org/10.3390/plants11070946
Chicago/Turabian StyleWang, Ya-Jie, Xing-Hou Zhen, Yang-Jiao Zhou, Yun-Lin Wang, Jing-Yi Hou, Xin Wang, Rui-Mei Li, Jiao Liu, Xin-Wen Hu, Meng-Ting Geng, and et al. 2022. "MeNINV1: An Alkaline/Neutral Invertase Gene of Manihot esculenta, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic Arabidopsis" Plants 11, no. 7: 946. https://doi.org/10.3390/plants11070946