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Communication

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

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Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic
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Department of Genetics and Agricultural Biotechnology, Faculty of Agriculture, University of South Bohemia, Studentská 1668, 370 05 České Budějovice, Czech Republic
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Research Institute of Oilseed Crops, OSEVA PRO. Ltd., Purkyňova 10, 764 01 Opava, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editors: Mohammad Reza Hajirezaei, Néstor Carrillo and Fahimeh Shahinnia
Plants 2021, 10(9), 1878; https://doi.org/10.3390/plants10091878
Received: 29 July 2021 / Revised: 30 August 2021 / Accepted: 8 September 2021 / Published: 10 September 2021
Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages. View Full-Text
Keywords: opium poppy; Papaver somniferum; drought response; transcriptomics; proteomics; dehydrins; gene expression; plant stress opium poppy; Papaver somniferum; drought response; transcriptomics; proteomics; dehydrins; gene expression; plant stress
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MDPI and ACS Style

Kundrátová, K.; Bartas, M.; Pečinka, P.; Hejna, O.; Rychlá, A.; Čurn, V.; Červeň, J. Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination. Plants 2021, 10, 1878. https://doi.org/10.3390/plants10091878

AMA Style

Kundrátová K, Bartas M, Pečinka P, Hejna O, Rychlá A, Čurn V, Červeň J. Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination. Plants. 2021; 10(9):1878. https://doi.org/10.3390/plants10091878

Chicago/Turabian Style

Kundrátová, Kristýna, Martin Bartas, Petr Pečinka, Ondřej Hejna, Andrea Rychlá, Vladislav Čurn, and Jiří Červeň. 2021. "Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination" Plants 10, no. 9: 1878. https://doi.org/10.3390/plants10091878

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