Conformation and Aggregation of Human Serum Albumin in the Presence of Green Tea Polyphenol (EGCg) and/or Palmitic Acid
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. HSA Labeled with CPM
2.3. HSA Labeled with Prodan
2.4. Reaction Mixtures
2.5. FRET Measurement
2.6. CD Measurements
2.7. DLS Measurements
3. Results
3.1. Interdomain Distances
3.2. Secondary Structure
3.3. Protein Size and Aggregation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sun, X.; Ferguson, H.N.; Hagerman, A.E. Conformation and Aggregation of Human Serum Albumin in the Presence of Green Tea Polyphenol (EGCg) and/or Palmitic Acid. Biomolecules 2019, 9, 705. https://doi.org/10.3390/biom9110705
Sun X, Ferguson HN, Hagerman AE. Conformation and Aggregation of Human Serum Albumin in the Presence of Green Tea Polyphenol (EGCg) and/or Palmitic Acid. Biomolecules. 2019; 9(11):705. https://doi.org/10.3390/biom9110705
Chicago/Turabian StyleSun, Xiaowei, Haley N. Ferguson, and Ann E. Hagerman. 2019. "Conformation and Aggregation of Human Serum Albumin in the Presence of Green Tea Polyphenol (EGCg) and/or Palmitic Acid" Biomolecules 9, no. 11: 705. https://doi.org/10.3390/biom9110705