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Open AccessArticle

An Insight into Storage Lipid Synthesis by Yarrowia lipolytica Yeast Relating to Lipid and Sugar Substrates Metabolism

1
Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, 02-776 Warsaw, Poland
2
Postgraduate School of Molecular Medicine, Medical University of Warsaw, 02-091 Warsaw, Poland
3
Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 685; https://doi.org/10.3390/biom9110685
Received: 11 September 2019 / Revised: 25 October 2019 / Accepted: 28 October 2019 / Published: 1 November 2019
(This article belongs to the Section Biochemistry)
Single cell oil (SCO) is the lipid accumulated in the cells of oleaginous microorganisms. Cellular lipids can be synthesized in two different pathways: de novo by metabolizing hydrophilic substrates and ex novo by fermenting hydrophobic substrates. The aim of the study was to evaluate the effect of carbon source (glucose and olive oil) in the culture medium on the course of microbial oil accumulation in Y. lipolytica cells. The level of selected gene expression by real time quantitative PCR method was investigated. The significant increase in expression of the POX2 gene encoding acyl-CoA oxidase II, which preferentially oxidizes long-chain acyl-CoAs formed from substrate fatty acids incorporated inside the microbial cell, was observed in medium with olive oil in relation to glucose containing medium. Noteworthily, the presence of lipid carbon substrate did not inhibit the level of ACL gene transcription coding for ATP-citrate lyase, the key enzyme of the lipid de novo accumulation process. The present study indicated that de novo lipid biosynthesis could occur despite the presence of fatty acids in the medium, and the synthesis of storage lipids in the presence of lipid carbon substrates could be carried out with the use of both pathways (de novo and ex novo). View Full-Text
Keywords: ACL gene; acyl-CoA oxidase; ATP-citrate lyase; POX2 gene; single cell oil; Yarrowia lipolytica ACL gene; acyl-CoA oxidase; ATP-citrate lyase; POX2 gene; single cell oil; Yarrowia lipolytica
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Fabiszewska, A.; Misiukiewicz-Stępień, P.; Paplińska-Goryca, M.; Zieniuk, B.; Białecka-Florjańczyk, E. An Insight into Storage Lipid Synthesis by Yarrowia lipolytica Yeast Relating to Lipid and Sugar Substrates Metabolism. Biomolecules 2019, 9, 685.

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