Biomolecules 2018, 8(2), 15; https://doi.org/10.3390/biom8020015
An Arabidopsis Clathrin Assembly Protein with a Predicted Role in Plant Defense Can Function as an Adenylate Cyclase
Patience Chatukuta 1
, Tshegofatso B. Dikobe 1, David T. Kawadza 1, Katlego S. Sehlabane 1, Mutsa M. Takundwa 1, Aloysius Wong 2, Chris Gehring 3 and Oziniel Ruzvidzo 1,*
, Tshegofatso B. Dikobe 1, David T. Kawadza 1, Katlego S. Sehlabane 1, Mutsa M. Takundwa 1, Aloysius Wong 2, Chris Gehring 3 and Oziniel Ruzvidzo 1,*
1
Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa
2
College of Natural, Applied and Health Sciences, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, Zhejiang Province, China
3
Department of Chemistry, Biology & Biotechnology, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy
*
Author to whom correspondence should be addressed.
Received: 18 December 2017 / Revised: 15 March 2018 / Accepted: 16 March 2018 / Published: 23 March 2018
Abstract
Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP261–379) can produce cyclic adenosine 3′,5′ monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization. View Full-TextKeywords:
Arabidopsis thaliana; adenylate cyclase; cAMP; clathrin assembly; endocytosis; pathogen responses
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Chatukuta, P.; Dikobe, T.B.; Kawadza, D.T.; Sehlabane, K.S.; Takundwa, M.M.; Wong, A.; Gehring, C.; Ruzvidzo, O. An Arabidopsis Clathrin Assembly Protein with a Predicted Role in Plant Defense Can Function as an Adenylate Cyclase. Biomolecules 2018, 8, 15.
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