Next Article in Journal
In Vivo Healthy Benefits of Galacto-Oligosaccharides from Lupinus albus (LA-GOS) in Butyrate Production through Intestinal Microbiota
Next Article in Special Issue
A Time-Kill Assay Study on the Synergistic Bactericidal Activity of Pomegranate Rind Extract and Zn (II) against Methicillin-Resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa
Previous Article in Journal
Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in Lentinula edodes Cultivated on Log versus Sawdust Substrates
Previous Article in Special Issue
Anticoronavirus and Immunomodulatory Phenolic Compounds: Opportunities and Pharmacotherapeutic Perspectives
 
 
Article

Glucosinolate Bioactivation by Apis mellifera Workers and Its Impact on Nosema ceranae Infection at the Colony Level

1
Research Centre for Cereal and Industrial Crops (CREA-CI), Council for Agricultural Research and Agricultural Economics Analysis, Via di Corticella 133, 40128 Bologna, Italy
2
Research Centre for Agriculture and Environment (CREA-AA), Council for Agricultural Research and Agricultural Economics Analysis, Via di Saliceto 80, 40128 Bologna, Italy
*
Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Academic Editor: Vicente Rubio
Biomolecules 2021, 11(11), 1657; https://doi.org/10.3390/biom11111657
Received: 5 October 2021 / Revised: 29 October 2021 / Accepted: 6 November 2021 / Published: 8 November 2021
(This article belongs to the Special Issue Bioactive Natural Compounds against Animal and Human Pathogens)
The microsporidian fungus Nosema ceranae represents one of the primary bee infection threats worldwide and the antibiotic fumagillin is the only registered product for nosemosis disease control, while few alternatives are, at present, available. Natural bioactive compounds deriving from the glucosinolate–myrosinase system (GSL–MYR) in Brassicaceae plants, mainly isothiocyanates (ITCs), are known for their antimicrobial activity against numerous pathogens and for their health-protective effects in humans. This work explored the use of Brassica nigra and Eruca sativa defatted seed meal (DSM) GSL-containing diets against natural Nosema infection in Apis mellifera colonies. DSM patties from each plant species were obtained by adding DSMs to sugar candy at the concentration of 4% (w/w). The feeding was administered in May to mildly N. ceranae-infected honey bee colonies for four weeks at the dose of 250 g/week. In the treated groups, no significant effects on colony development and bee mortality were observed compared to the negative controls. The N. ceranae abundance showed a slight but significant decrease. Furthermore, the GSL metabolism in bees was investigated, and MYR hydrolytic activity was qualitatively searched in isolated bee midgut and hindgut. Interestingly, MYR activity was detected both in the bees fed DSMs and in the control group where the bees did not receive DSMs. In parallel, ITCs were found in gut tissues from the bees treated with DSMs, corroborating the presence of a MYR-like enzyme capable of hydrolyzing ingested GSLs. On the other hand, GSLs and other GSL hydrolysis products other than ITCs, such as nitriles, were found in honey produced by the treated bees, potentially increasing the health value of the final product for human consumption. The results are indicative of a specific effect on the N. ceranae infection in managed honey bee colonies depending on the GSL activation within the target organ. View Full-Text
Keywords: Brassicaceae; Brassica nigra; Eruca sativa; nosemosis; honey bee; field trials; isothiocyanate; myrosinase; honey Brassicaceae; Brassica nigra; Eruca sativa; nosemosis; honey bee; field trials; isothiocyanate; myrosinase; honey
Show Figures

Figure 1

MDPI and ACS Style

Ugolini, L.; Cilia, G.; Pagnotta, E.; Malaguti, L.; Capano, V.; Guerra, I.; Zavatta, L.; Albertazzi, S.; Matteo, R.; Lazzeri, L.; Righetti, L.; Nanetti, A. Glucosinolate Bioactivation by Apis mellifera Workers and Its Impact on Nosema ceranae Infection at the Colony Level. Biomolecules 2021, 11, 1657. https://doi.org/10.3390/biom11111657

AMA Style

Ugolini L, Cilia G, Pagnotta E, Malaguti L, Capano V, Guerra I, Zavatta L, Albertazzi S, Matteo R, Lazzeri L, Righetti L, Nanetti A. Glucosinolate Bioactivation by Apis mellifera Workers and Its Impact on Nosema ceranae Infection at the Colony Level. Biomolecules. 2021; 11(11):1657. https://doi.org/10.3390/biom11111657

Chicago/Turabian Style

Ugolini, Luisa, Giovanni Cilia, Eleonora Pagnotta, Lorena Malaguti, Vittorio Capano, Irene Guerra, Laura Zavatta, Sergio Albertazzi, Roberto Matteo, Luca Lazzeri, Laura Righetti, and Antonio Nanetti. 2021. "Glucosinolate Bioactivation by Apis mellifera Workers and Its Impact on Nosema ceranae Infection at the Colony Level" Biomolecules 11, no. 11: 1657. https://doi.org/10.3390/biom11111657

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop