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Open AccessArticle

Metabolic Effects of Dietary Glycerol Supplementation in Muscle and Liver of European Seabass and Rainbow Trout by 1H NMR Metabolomics

1
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal
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Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal
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Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal
4
CIIMAR—Interdisciplinary Centre of Marine and Environmental Research—University of Porto, 4050-123 Porto, Portugal
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(10), 202; https://doi.org/10.3390/metabo9100202
Received: 14 August 2019 / Revised: 20 September 2019 / Accepted: 22 September 2019 / Published: 27 September 2019
(This article belongs to the Special Issue Metabolomics 2019)
The sustainable growth of fish aquaculture will require the procurement of non-marine feed sources. Glycerol is a potential feed supplement whose metabolism may spare the catabolism of dietary amino acids, thereby extending the use of the feed protein to other physiological functions such as growth. In the present study, the effects of dietary glycerol supplementation on the muscle and liver metabolomes of rainbow trout (Oncorhynchus mykiss) and European seabass (Dicentrarchus labrax) were evaluated. Fish juveniles were fed diets with 0%, 2.5%, and 5% glycerol. Muscle and liver aqueous fractions were extracted and 1H NMR spectra were acquired. Metabolite profiles derived from the 1H NMR signals were assessed using univariate and multivariate statistical analyses. The adenylate energy charge was determined in the muscle. For both species, the muscle metabolite profile showed more variability compared to that of the liver and was most perturbed by the 5.0% glycerol diet. For the liver metabolite profile, rainbow trout showed fewer differences compared to European seabass. No differences were observed in energy charge between experimental groups for either species. Thus, rainbow trout appeared to be less susceptible to tissue metabolite perturbations, compared to seabass, when the diet was supplemented with up to 5% glycerol. View Full-Text
Keywords: aquaculture; aquafeed; diet optimization; fish metabolism aquaculture; aquafeed; diet optimization; fish metabolism
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MDPI and ACS Style

Palma, M.; Tavares, L.C.; Rito, J.; Henriques, L.F.; Silva, J.G.; Ozório, R.; Pardal, M.A.; Magnoni, L.J.; Viegas, I. Metabolic Effects of Dietary Glycerol Supplementation in Muscle and Liver of European Seabass and Rainbow Trout by 1H NMR Metabolomics. Metabolites 2019, 9, 202.

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