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Article

Respiratory Parameters for the Classification of Dysfunctional Insulin Secretion by Pancreatic Islets

1
Division of Metabolic Diseases, Technische Universität München, 80333 Munich, Germany
2
Department of Pharmaceutical Chemistry, Parul Institute of Pharmacy, Parul University, Vadodara 391760, India
3
Faculty of Health, School of Biomedical Sciences, University of Plymouth, Plymouth Science Park, Plymouth PL6 8BU, UK
4
Department of Molecular Biosciences, The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, SE-106 91 Stockholm, Sweden
5
Helmholtz Diabetes Center, Institute for Diabetes and Obesity, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Melkam Kebede and Belinda Yau
Metabolites 2021, 11(6), 405; https://doi.org/10.3390/metabo11060405
Received: 3 June 2021 / Revised: 17 June 2021 / Accepted: 18 June 2021 / Published: 21 June 2021
(This article belongs to the Special Issue Islet Biology and Metabolism)
The development of obesity and type 2 diabetes (T2D) has been associated with impaired mitochondrial function. In pancreatic beta (β) cells, mitochondrial energy metabolism plays a central role in triggering and controlling glucose-stimulated insulin secretion (GSIS). Here, we have explored whether mitochondrial bioenergetic parameters assessed with Seahorse extracellular flux technology can quantitatively predict insulin secretion. We metabolically stressed male C57BL/6 mice by high-fat feeding (HFD) and measured the glucose sensitivity of islet respiration and insulin secretion. The diet-induced obese (DIO) mice developed hyperinsulinemia, but no pathological secretory differences were apparent between isolated DIO and chow islets. Real-time extracellular flux analysis, however, revealed a lower respiratory sensitivity to glucose in DIO islets. Correlation of insulin secretion with respiratory parameters uncovers compromised insulin secretion in DIO islets by oxidative power. Normalization to increased insulin contents during DIO improves the quantitative relation between GSIS and respiration, allowing to classify dysfunctional properties of pancreatic insulin secretion, and thereby serving as valuable biomarker for pancreatic islet glucose responsiveness and health. View Full-Text
Keywords: mitochondria; bioenergetics; glucose-stimulated insulin secretion; pancreatic islets; respiration mitochondria; bioenergetics; glucose-stimulated insulin secretion; pancreatic islets; respiration
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MDPI and ACS Style

Kabra, U.D.; Affourtit, C.; Jastroch, M. Respiratory Parameters for the Classification of Dysfunctional Insulin Secretion by Pancreatic Islets. Metabolites 2021, 11, 405. https://doi.org/10.3390/metabo11060405

AMA Style

Kabra UD, Affourtit C, Jastroch M. Respiratory Parameters for the Classification of Dysfunctional Insulin Secretion by Pancreatic Islets. Metabolites. 2021; 11(6):405. https://doi.org/10.3390/metabo11060405

Chicago/Turabian Style

Kabra, Uma D., Charles Affourtit, and Martin Jastroch. 2021. "Respiratory Parameters for the Classification of Dysfunctional Insulin Secretion by Pancreatic Islets" Metabolites 11, no. 6: 405. https://doi.org/10.3390/metabo11060405

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