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Exploratory Analysis of Commercial Olive-Based Dietary Supplements Using Untargeted and Targeted Metabolomics

Untargeted Metabolomics Studies on Drug-Incubated Phragmites australis Profiles

Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748 Garching, Germany
German Research Center for Environmental Health, Research Unit Comparative Microbiome Analysis, Helmholtz Centrum Munich, Ingolstadt Street 1, 85764 Neuherberg, Germany
Stefan Moser Process Optimization, Weberweg 3, D-83131 Nußdorf am Inn, Germany
Analytisches Forschungsinstitut für Non-Target Screening GmbH (AFIN-TS GmbH), Am Mittleren Moos 48, D-86167 Augsburg, Germany
Author to whom correspondence should be addressed.
Current address: HydroSciences Montpellier, UMR 5569, Faculté de Pharmacie, University of Montpellier, Avenue Charles Flahault 15, 34000 Montpellier, France.
Metabolites 2021, 11(1), 2;
Received: 12 November 2020 / Revised: 20 December 2020 / Accepted: 21 December 2020 / Published: 22 December 2020
(This article belongs to the Special Issue Plant and Derived Natural Product Metabolomics)
Plants produce a huge number of functionally and chemically different natural products that play an important role in linking the plant with the adjacent environment. Plants can also absorb and transform external organic compounds (xenobiotics). Currently there are only a few studies concerning the effects of xenobiotics and their transformation products on plant metabolites using a mass spectrometric untargeted screening strategy. This study was designed to investigate the changes of the Phragmites australis metabolome following/after diclofenac or carbamazepine incubation, using a serial coupling of reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) combined with accurate high-resolution time-of-flight mass spectrometer (TOF-MS). An untargeted screening strategy of metabolic fingerprints was developed to purposefully compare samples from differently treated P. australis plants, revealing that P. australis responded to each drug differently. When solvents with significantly different polarities were used, the metabolic profiles of P. australis were found to change significantly. For instance, the production of polyphenols (such as quercetin) in the plant increased after diclofenac incubation. Moreover, the pathway of unsaturated organic acids became more prominent, eventually as a reaction to protect the cells against reactive oxygen species (ROS). Hence, P. australis exhibited an adaptive mechanism to cope with each drug. Consequently, the untargeted screening approach is essential for understanding the complex response of plants to xenobiotics. View Full-Text
Keywords: P. australis metabolic profile; untargeted metabolomics; diclofenac; carbamazepine; orthogonal partial least square-discriminant analysis (OPLS-DA) P. australis metabolic profile; untargeted metabolomics; diclofenac; carbamazepine; orthogonal partial least square-discriminant analysis (OPLS-DA)
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MDPI and ACS Style

Wahman, R.; Sauvêtre, A.; Schröder, P.; Moser, S.; Letzel, T. Untargeted Metabolomics Studies on Drug-Incubated Phragmites australis Profiles. Metabolites 2021, 11, 2.

AMA Style

Wahman R, Sauvêtre A, Schröder P, Moser S, Letzel T. Untargeted Metabolomics Studies on Drug-Incubated Phragmites australis Profiles. Metabolites. 2021; 11(1):2.

Chicago/Turabian Style

Wahman, Rofida, Andres Sauvêtre, Peter Schröder, Stefan Moser, and Thomas Letzel. 2021. "Untargeted Metabolomics Studies on Drug-Incubated Phragmites australis Profiles" Metabolites 11, no. 1: 2.

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