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Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines

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Department of Pharmacology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
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Department of Chemistry, Faculty of Mathematics and Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
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Faculty of Medicine, Universitas Padjadjaran, Bandung 40161, Indonesia
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Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
*
Author to whom correspondence should be addressed.
Academic Editor: Helen D. Skaltsa
Sci. Pharm. 2021, 89(2), 21; https://doi.org/10.3390/scipharm89020021
Received: 9 April 2021 / Revised: 11 May 2021 / Accepted: 17 May 2021 / Published: 19 May 2021
Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the secondary metabolites using ethyl acetate from its root bark and further tested its antiproliferative activities against P388 murine leukemia cell lines. The subfractions from the ethyl acetate extract was obtained from Vacuum Liquid Column Chromatography (VLCC), and followed by Gravity Column Chromatography (GCC). The subfraction C2 and D1 were identified to contain triterpenoids and steroids with the most potent cytotoxicity against Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that ethyl acetate extract has the highest antiproliferative activities against P388 murine leukemia cell lines (IC50 = 21.79 μg/mL), as opposed to subfraction C2 (IC50 = 50.64 µg/mL) and subfraction D1 (IC50 = 49.33 µg/mL). The compound identified in subfraction C2 and D1 are taraxerol acetate and calotropone, respectively. Though taraxerol acetate and calotropone were active in inhibiting the leukemic cell lines, their IC50s were lower than the ethyl acetate extract, which is probably due to the synergism of the secondary metabolites. View Full-Text
Keywords: leukemia; calotropone; taraxerol acetate; anticancer; Calotropis gigantea leukemia; calotropone; taraxerol acetate; anticancer; Calotropis gigantea
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MDPI and ACS Style

Hasballah, K.; Sarong, M.; Rusly, R.; Fitria, H.; Maida, D.R.; Iqhrammullah, M. Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines. Sci. Pharm. 2021, 89, 21. https://doi.org/10.3390/scipharm89020021

AMA Style

Hasballah K, Sarong M, Rusly R, Fitria H, Maida DR, Iqhrammullah M. Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines. Scientia Pharmaceutica. 2021; 89(2):21. https://doi.org/10.3390/scipharm89020021

Chicago/Turabian Style

Hasballah, Kartini; Sarong, Murniana; Rusly, Renzavaldy; Fitria, Herdina; Maida, Dewi R.; Iqhrammullah, Muhammad. 2021. "Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines" Sci. Pharm. 89, no. 2: 21. https://doi.org/10.3390/scipharm89020021

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