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Article

Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria

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Instituto Tecnológico de Celaya, Campus I, Antonio García Cubas Pte. #600 esq. Av. Tecnológico, Celaya C. 3810, Mexico
2
Área Académica de Química, Instituto de Ciencias Básicas e Ingeniería, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma C.P 42183, Mexico
*
Author to whom correspondence should be addressed.
Antibiotics 2019, 8(4), 218; https://doi.org/10.3390/antibiotics8040218
Received: 11 October 2019 / Revised: 3 November 2019 / Accepted: 4 November 2019 / Published: 11 November 2019
(This article belongs to the Special Issue Natural Compounds as Antimicrobial Agents)
The anti-microbial properties of acetone extracts from Hibiscus sabdariffa calyces, fractions isolated by silica gel chromatography and hibiscus acid purified from some of these fractions and additionally identified by nuclear magnetic resonance spectroscopy, mid-infrared spectroscopy and X-ray diffraction, were studied against both multidrug-resistant Salmonella strains and pathogenic Escherichia coli bacteria. Gel diffusion was used to determine the anti-microbial effects. The mode of action of hibiscus acid was determined by crystal violet assay. Hibiscus acid and 17 of the 25 chromatographic fractions obtained, displayed an anti-microbial effect against all bacterial strains tested. Hibiscus acid showed a greater anti-microbial effect than the acetone extract against most of the bacteria strains, while chromatographic fractions IX–XIV exerted the greatest anti-microbial effect against all bacteria. The minimum inhibitory concentration of the acetone extract was 7 mg/mL, and the minimum bactericidal concentration was 10 mg/mL, while the corresponding values for hibiscus acid were 4–7 and 7 mg/mL, respectively. The results of the crystal violet assay indicate that hibiscus acid alters membrane permeability. Hibiscus acid is a potential alternative to control multidrug-resistant bacteria. Due to its ready availability and easy extraction from H. sabdariffa, hibiscus acid is potentially useful in the food industries. View Full-Text
Keywords: anti-microbial; Hibiscus acid; Hibiscus sabdariffa; multidrug-resistant; antibiotic anti-microbial; Hibiscus acid; Hibiscus sabdariffa; multidrug-resistant; antibiotic
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MDPI and ACS Style

Portillo-Torres, L.A.; Bernardino-Nicanor, A.; Gómez-Aldapa, C.A.; González-Montiel, S.; Rangel-Vargas, E.; Villagómez-Ibarra, J.R.; González-Cruz, L.; Cortés-López, H.; Castro-Rosas, J. Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria. Antibiotics 2019, 8, 218. https://doi.org/10.3390/antibiotics8040218

AMA Style

Portillo-Torres LA, Bernardino-Nicanor A, Gómez-Aldapa CA, González-Montiel S, Rangel-Vargas E, Villagómez-Ibarra JR, González-Cruz L, Cortés-López H, Castro-Rosas J. Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria. Antibiotics. 2019; 8(4):218. https://doi.org/10.3390/antibiotics8040218

Chicago/Turabian Style

Portillo-Torres, Lizbeth A., Aurea Bernardino-Nicanor, Carlos A. Gómez-Aldapa, Simplicio González-Montiel, Esmeralda Rangel-Vargas, José R. Villagómez-Ibarra, Leopoldo González-Cruz, Humberto Cortés-López, and Javier Castro-Rosas. 2019. "Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria" Antibiotics 8, no. 4: 218. https://doi.org/10.3390/antibiotics8040218

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