In Vitro Activity of Nisin A Against Staphylococci Isolated from Periprosthetic Joint Infection
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
PJI | Periprosthetic joint infection |
MRSA | Methicillin-resistant Staphylococcus aureus |
MSSA | Methicillin-susceptible Staphylococcus aureus |
MRSE | Methicillin-resistant Staphylococcus epidermidis |
MSSE | Methicillin-susceptible Staphylococcus epidermidis |
CaMHB | Cation-adjusted Mueller Hinton broth |
MIC | Minimum inhibitory concentration |
MBBC | Minimum biofilm bactericidal concentration |
PBS | Phosphate-buffered saline |
FIC | Fractional inhibitory concentration |
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MIC (µg/mL) | MIC50 | MIC90 | ||||||||||
≤0.5 | 1 | 2 | 4 | 8 | 16 | 32 | 64 | 128 | 256 | |||
MRSA | 7 (26.9) | 15 (57.7) | 4 (15.4) | 4 | 8 | |||||||
MSSA | 11 (37.9) | 17 (58.6) | 1 (3.5) | 4 | 4 | |||||||
MRSE | 4 (14.8) | 17 (63) | 5 (18.5) | 1 (3.7) | 2 | 4 | ||||||
MSSE | 1 (9.1) | 7 (63.6) | 3 (27.3) | 2 | 4 | |||||||
S. lug | 2 (28.6) | 3 (42.8) | 2 (28.6) | NA | NA | |||||||
Other | 4 (66.7) | 2 (33.3) | NA | NA | ||||||||
MBIC (µg/mL) | MBIC50 | MBIC90 | ||||||||||
≤0.5 | 1 | 2 | 4 | 8 | 16 | 32 | 64 | 128 | 256 | |||
MRSA | 1 (3.8) | 9 (34.6) | 14 (53.8) | 2 (7.7) | 4 | 4 | ||||||
MSSA | 10 (34.5) | 10 (34.5) | 8 (27.5) | 1 (3.5) | 2 | 4 | ||||||
MRSE | 2 (7.4) | 8 (29.7) | 13 (48.1) | 2 (7.4) | 2 (7.4) | 2 | 4 | |||||
MSSE | 1 (9.1) | 5 (45.4) | 4 (36.4) | 1 (9.1) | 2 | 4 | ||||||
S. lug | 2 (28.6) | 5 (74.1) | NA | NA | ||||||||
Other | 3 (50) | 2 (33.3) | 1 (16.7) | NA | NA | |||||||
MBBC (µg/mL) | MBBC50 | MBBC90 | ||||||||||
≤0.5 | 1 | 2 | 4 | 8 | 16 | 32 | 64 | 128 | 256 | |||
MRSA | 1 (3.8) | 6 (23.1) | 10 (38.5) | 5 (19.2) | 4 (15.4) | 64 | 256 | |||||
MSSA | 1 (3.5) | 3 (10.3) | 18 (62.1) | 5 (17.2) | 1 (3.5) | 1 (3.5) | 32 | 64 | ||||
MRSE | 2 (7.4) | 7 (25.9) | 13 (48.1) | 5 (18.5) | 64 | 128 | ||||||
MSSE | 2 (18.2) | 6 (54.5) | 2 (18.2) | 1 (9.1) | 64 | 128 | ||||||
S. lug | 2 (28.6) | 2 (28.6) | 2 (28.6) | 1 (14.2) | NA | NA | ||||||
Other | 1 (16.7) | 3 (50) | 2 (33.3) | NA | NA |
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Karau, M.J.; Koscianski, C.A.; Badley, A.D.; Bedard, N.A.; Zinckgraf, J.W.; Patel, R. In Vitro Activity of Nisin A Against Staphylococci Isolated from Periprosthetic Joint Infection. Antibiotics 2025, 14, 515. https://doi.org/10.3390/antibiotics14050515
Karau MJ, Koscianski CA, Badley AD, Bedard NA, Zinckgraf JW, Patel R. In Vitro Activity of Nisin A Against Staphylococci Isolated from Periprosthetic Joint Infection. Antibiotics. 2025; 14(5):515. https://doi.org/10.3390/antibiotics14050515
Chicago/Turabian StyleKarau, Melissa J., Christina A. Koscianski, Andrew D. Badley, Nicholas A. Bedard, John W. Zinckgraf, and Robin Patel. 2025. "In Vitro Activity of Nisin A Against Staphylococci Isolated from Periprosthetic Joint Infection" Antibiotics 14, no. 5: 515. https://doi.org/10.3390/antibiotics14050515
APA StyleKarau, M. J., Koscianski, C. A., Badley, A. D., Bedard, N. A., Zinckgraf, J. W., & Patel, R. (2025). In Vitro Activity of Nisin A Against Staphylococci Isolated from Periprosthetic Joint Infection. Antibiotics, 14(5), 515. https://doi.org/10.3390/antibiotics14050515