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Article

An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis

1
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, China
2
Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China
3
Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
*
Authors to whom correspondence should be addressed.
Antibiotics 2021, 10(5), 603; https://doi.org/10.3390/antibiotics10050603
Submission received: 14 February 2021 / Revised: 6 March 2021 / Accepted: 10 March 2021 / Published: 19 May 2021
(This article belongs to the Special Issue Antibiotic Resistance and Treatment of MRSA Infection)

Abstract

Staphylococcus aureus (S. aureus) is one of most common opportunistic pathogens and is attributed to several human infections. The increasing incidence of methicillin-resistant S. aureus (MRSA) is a serious clinical threat for osteomyelitis crisis. The YycFG two-component system of S. aureus regulates genes associated with biofilm formation. To investigate the potential role of an antisense yycF RNA in the regulation of transcription levels of yycF and associated effects on biofilm formation and pathogenicity, antisense yycF (ASyycF) RNA was detected by RT-PCR and 5′ RACE assays. ASyycF overexpression mutants were constructed, and the biofilm biomass was determined by crystal violet microtiter assay and scanning electron microscopy (SEM). Quantitative RT-PCR and Western blotting analyses were used to detect whether ASyycF overexpression inhibited the transcription and translation of biofilm-related genes. Then, a rat tibial infective model was used to evaluate the pathogenicity of ASyycF overexpression in vivo. ASyycF transcription led to reductions in YycF production and biofilm formation. Overexpression of ASyycF inhibited the transcription and translation of biofilm-related genes. The sensitivity to vancomycin was improved in ASyycF-overexpressing MRSA. Furthermore, ASyycF inhibited MRSA invasion in a rat tibial infection model. From this study, the expression of the YycF protein was found to be inversely correlated with different levels of ASyycF transcription. The biofilm biomass and pathogenicity decreased in the ASyycF-overexpressing mutant. Thus, the current evidence may support ASyycF as a supplementary strategy for managing S. aureus and MRSA infections.
Keywords: antisense; biofilm formation; Staphylococcus aureus; YycFG; osteomyelitis antisense; biofilm formation; Staphylococcus aureus; YycFG; osteomyelitis

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MDPI and ACS Style

Wu, S.; Liu, Y.; Lei, L.; Zhang, H. An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis. Antibiotics 2021, 10, 603. https://doi.org/10.3390/antibiotics10050603

AMA Style

Wu S, Liu Y, Lei L, Zhang H. An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis. Antibiotics. 2021; 10(5):603. https://doi.org/10.3390/antibiotics10050603

Chicago/Turabian Style

Wu, Shizhou, Yunjie Liu, Lei Lei, and Hui Zhang. 2021. "An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis" Antibiotics 10, no. 5: 603. https://doi.org/10.3390/antibiotics10050603

APA Style

Wu, S., Liu, Y., Lei, L., & Zhang, H. (2021). An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis. Antibiotics, 10(5), 603. https://doi.org/10.3390/antibiotics10050603

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