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Article

Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers

1
Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain
2
Departamento de Bioquímica y Biología Molecular, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Luigi Campanella
Biosensors 2016, 6(4), 56; https://doi.org/10.3390/bios6040056
Received: 27 September 2016 / Revised: 4 November 2016 / Accepted: 14 November 2016 / Published: 21 November 2016
(This article belongs to the Special Issue Next-Generation Immunosensors)
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation. View Full-Text
Keywords: human p53; magnetic microcarriers; screen-printed electrodes; amperometric immunosensor; cell lysates human p53; magnetic microcarriers; screen-printed electrodes; amperometric immunosensor; cell lysates
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MDPI and ACS Style

Pedrero, M.; Manuel de Villena, F.J.; Muñoz-San Martín, C.; Campuzano, S.; Garranzo-Asensio, M.; Barderas, R.; Pingarrón, J.M. Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers. Biosensors 2016, 6, 56. https://doi.org/10.3390/bios6040056

AMA Style

Pedrero M, Manuel de Villena FJ, Muñoz-San Martín C, Campuzano S, Garranzo-Asensio M, Barderas R, Pingarrón JM. Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers. Biosensors. 2016; 6(4):56. https://doi.org/10.3390/bios6040056

Chicago/Turabian Style

Pedrero, María, F. J. Manuel de Villena, Cristina Muñoz-San Martín, Susana Campuzano, María Garranzo-Asensio, Rodrigo Barderas, and José M. Pingarrón. 2016. "Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers" Biosensors 6, no. 4: 56. https://doi.org/10.3390/bios6040056

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