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Kinetics of Antibody Binding to Membranes of Living Bacteria Measured by a Photonic Crystal-Based Biosensor

Laboratoire de Physique de la Matière Vivante, IPHYS, École Polytechnique Fédérale de Lausanne (EPFL); Rte de la Sorge, 1015 Lausanne, Switzerland
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Academic Editors: Olga Korostynska and Alex Mason
Biosensors 2016, 6(4), 52; https://doi.org/10.3390/bios6040052
Received: 11 July 2016 / Revised: 31 August 2016 / Accepted: 28 September 2016 / Published: 11 October 2016
Optical biosensors based on photonic crystal surface waves (PC SWs) offer a possibility to study binding interactions with living cells, overcoming the limitation of rather small evanescent field penetration depth into a sample medium that is characteristic for typical optical biosensors. Besides this, simultaneous excitation of s- and p-polarized surface waves with different penetration depths is realized here, permitting unambiguous separation of surface and volume contributions to the measured signal. PC-based biosensors do not require a bulk signal correction, compared to widely used surface plasmon resonance-based devices. We developed a chitosan-based protocol of PC chip functionalization for bacterial attachment and performed experiments on antibody binding to living bacteria measured in real time by the PCSW-based biosensor. Data analysis reveals specific binding and gives the value of the dissociation constant for monoclonal antibodies (IgG2b) against bacterial lipopolysaccharides equal to KD = 6.2 ± 3.4 nM. To our knowledge, this is a first demonstration of antibody-binding kinetics to living bacteria by a label-free optical biosensor. View Full-Text
Keywords: photonic crystal; label-free biosensor; bacteria; binding kinetics; antibody; ligand-receptor interaction photonic crystal; label-free biosensor; bacteria; binding kinetics; antibody; ligand-receptor interaction
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MDPI and ACS Style

Rostova, E.; Ben Adiba, C.; Dietler, G.; Sekatskii, S.K. Kinetics of Antibody Binding to Membranes of Living Bacteria Measured by a Photonic Crystal-Based Biosensor. Biosensors 2016, 6, 52. https://doi.org/10.3390/bios6040052

AMA Style

Rostova E, Ben Adiba C, Dietler G, Sekatskii SK. Kinetics of Antibody Binding to Membranes of Living Bacteria Measured by a Photonic Crystal-Based Biosensor. Biosensors. 2016; 6(4):52. https://doi.org/10.3390/bios6040052

Chicago/Turabian Style

Rostova, Ekaterina; Ben Adiba, Carine; Dietler, Giovanni; Sekatskii, Sergey K. 2016. "Kinetics of Antibody Binding to Membranes of Living Bacteria Measured by a Photonic Crystal-Based Biosensor" Biosensors 6, no. 4: 52. https://doi.org/10.3390/bios6040052

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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