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Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC 880 Rue de Mimet, Gardanne 13541, France
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 13 November 2012; in revised form: 15 December 2012 / Accepted: 31 December 2012 / Published: 8 January 2013
Abstract: Barrier tissue protects the body against external factors by restricting the passage of molecules. The gastrointestinal epithelium is an example of barrier tissue with the primary purpose of allowing the passage of ions and nutrients, while restricting the passage of pathogens and toxins. It is well known that the loss of barrier function can be instigated by a decrease in extracellular calcium levels, leading to changes in protein conformation and an increase in paracellular transport. In this study, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid (EGTA), a calcium chelator, was used to disrupt the gastrointestinal epithelial barrier. The effect of EGTA on barrier tissue was monitored by a novel label-free method based on an organic electrochemical transistor (OECT) integrated with living cells and validated against conventional methods for measuring barrier tissue integrity. We demonstrate that the OECT can detect breaches in barrier tissue upon exposure to EGTA with the same sensitivity as existing methods but with increased temporal resolution. Due to the potential of low cost processing techniques and the flexibility in design associated with organic electronics, the OECT has great potential for high-throughput, disposable sensing and diagnostics.
Keywords: organic bioelectronics; tight junctions; paracellular transport; EGTA; barrier tissue; toxicology; biosensing; organic electrochemical transistor
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MDPI and ACS Style
Tria, S.; Jimison, L.H.; Hama, A.; Bongo, M.; Owens, R.M. Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor. Biosensors 2013, 3, 44-57.
Tria S, Jimison LH, Hama A, Bongo M, Owens RM. Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor. Biosensors. 2013; 3(1):44-57.
Tria, Scherrine; Jimison, Leslie H.; Hama, Adel; Bongo, Manuelle; Owens, Róisín M. 2013. "Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor." Biosensors 3, no. 1: 44-57.