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Volume 15
Issue 12
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Article

An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume

by
Warisara Sretapunya
1,
Thitiya Buranachat
1,
Montita Prasomthong
1,
Rittichai Tantikorn
1,
Areerat Sa-ngarsang
2,
Sirirat Naemkhunthot
2,
Laddawan Meephaendee
2,
Pattara Wongjaroen
2,
Chika Tanaka
3,
Yoriko Shimadzu
3,
Katsuya Ogata
3,
Kunihiro Kaihatsu
3,
Ryo Morita
4,5,6,
Michinori Shirano
4,
Juthamas Phadungsombat
6,
Tadahiro Sasaki
6,
Ritsuko Kubota-Koketsu
6,
Yoshihiro Samune
6,
Emi E. Nakayama
6 and
Tatsuo Shioda
6,*
1
Nakhon Nayok Hospital, Nakhon Nayok 26000, Thailand
2
National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000, Thailand
3
VisGene Ltd., Osaka 562-0033, Japan
4
Department of Infectious Diseases, Osaka City General Hospital, Osaka 534-0021, Japan
5
Department of Infectious Diseases, Osaka City Juso Hospital, Osaka 532-0034, Japan
6
Department of Viral Infections, Research Institute for Microbial Diseases, The University of Osaka, Osaka 565-0871, Japan
*
Author to whom correspondence should be addressed.
Biosensors 2025, 15(12), 802; https://doi.org/10.3390/bios15120802 (registering DOI)
Submission received: 27 October 2025 / Revised: 4 December 2025 / Accepted: 5 December 2025 / Published: 7 December 2025
(This article belongs to the Special Issue Biosensors for Healthcare and Environment: Current and Future Perspectives)
Versions Notes

Abstract

The four serotypes of dengue virus (DENV), types 1 to 4 (DENV-1 to DENV-4), exhibit approximately 60% identity in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from patient serum specimens followed by PCR amplification with serotype-specific probes is the current standard technique for DENV serotyping. However, this method is time- and cost-consuming, and rapid detection systems with low cost are desirable. Previously, we developed a prototype serotype-specific immunochromatography system. That system was composed of four strips with four corresponding distinct sample buffers, each specifically detecting a single DENV serotype. In the present study, we improved this system by combining pairs of strips into one lateral-flow cassette each, providing DENV-1 and DENV-2 detection in one device and DENV-3 and DENV-4 detection in a second device; this strategy successfully reduced the required sample volume. Furthermore, we were able to adjust the composition of the sample buffers such that a single sample buffer sufficed for all four DENV serotype detection reactions, allowing much easier handling of the devices. Evaluation of this new device against laboratory and clinical DENV isolates and clinical specimens from DENV-infected individuals showed sensitivity that was comparable to that of our previous version, yielding serotype specificity of 100%. These new devices are expected to be of use in the clinical setting, accelerating both prospective and retrospective epidemiological studies.
Keywords: dengue virus; serotype discrimination; rapid test; immunochromatography; NS1 dengue virus; serotype discrimination; rapid test; immunochromatography; NS1

Share and Cite

MDPI and ACS Style

Sretapunya, W.; Buranachat, T.; Prasomthong, M.; Tantikorn, R.; Sa-ngarsang, A.; Naemkhunthot, S.; Meephaendee, L.; Wongjaroen, P.; Tanaka, C.; Shimadzu, Y.; et al. An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume. Biosensors 2025, 15, 802. https://doi.org/10.3390/bios15120802

AMA Style

Sretapunya W, Buranachat T, Prasomthong M, Tantikorn R, Sa-ngarsang A, Naemkhunthot S, Meephaendee L, Wongjaroen P, Tanaka C, Shimadzu Y, et al. An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume. Biosensors. 2025; 15(12):802. https://doi.org/10.3390/bios15120802

Chicago/Turabian Style

Sretapunya, Warisara, Thitiya Buranachat, Montita Prasomthong, Rittichai Tantikorn, Areerat Sa-ngarsang, Sirirat Naemkhunthot, Laddawan Meephaendee, Pattara Wongjaroen, Chika Tanaka, Yoriko Shimadzu, and et al. 2025. "An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume" Biosensors 15, no. 12: 802. https://doi.org/10.3390/bios15120802

APA Style

Sretapunya, W., Buranachat, T., Prasomthong, M., Tantikorn, R., Sa-ngarsang, A., Naemkhunthot, S., Meephaendee, L., Wongjaroen, P., Tanaka, C., Shimadzu, Y., Ogata, K., Kaihatsu, K., Morita, R., Shirano, M., Phadungsombat, J., Sasaki, T., Kubota-Koketsu, R., Samune, Y., Nakayama, E. E., & Shioda, T. (2025). An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume. Biosensors, 15(12), 802. https://doi.org/10.3390/bios15120802

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