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Fabrication, Characterization and Application of Biomolecule Micropatterns on Cyclic Olefin Polymer (COP) Surfaces with Adjustable Contrast

1
Center for Advanced Bioanalysis GmbH, Linz 4020, Austria
2
University of Applied Sciences Upper Austria, Stelzhamerstrasse 23, Wels A-4600, Austria
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(1), 3; https://doi.org/10.3390/bios10010003
Received: 17 November 2019 / Revised: 19 December 2019 / Accepted: 25 December 2019 / Published: 28 December 2019
Peptide and protein micropatterns are powerful tools for the investigation of various cellular processes, including protein–protein interactions (PPIs). Within recent years, various approaches for the production of functional surfaces have been developed. Most of these systems use glass as a substrate, which has several drawbacks, including high fragility and costs, especially if implemented for fluorescence microscopy. In addition, conventional fabrication technologies such as microcontact printing (µCP) are frequently used for the transfer of biomolecules to the glass surface. In this case, it is challenging to adjust the biomolecule density. Here, we show that cyclic olefin polymer (COP) foils, with their encouraging properties, including the ease of manufacturing, chemical resistance, biocompatibility, low water absorption, and optical clarity, are a promising alternative to glass substrates for the fabrication of micropatterns. Using a photolithography-based approach, we generated streptavidin/biotinylated antibody patterns on COPs with the possibility of adjusting the pattern contrast by varying plasma activation parameters. Our experimental setup was finally successfully implemented for the analysis of PPIs in the membranes of live cells via total internal reflection fluorescence (TIRF) microscopy. View Full-Text
Keywords: micropatterns; photolithography; protein–protein interaction; micropatterned polymers; cyclic olefin polymer; total internal reflection fluorescence (TIRF) micropatterns; photolithography; protein–protein interaction; micropatterned polymers; cyclic olefin polymer; total internal reflection fluorescence (TIRF)
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Hager, R.; Haselgrübler, T.; Haas, S.; Lipp, A.-M.; Weghuber, J. Fabrication, Characterization and Application of Biomolecule Micropatterns on Cyclic Olefin Polymer (COP) Surfaces with Adjustable Contrast. Biosensors 2020, 10, 3.

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