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Detection of Ampicillin-Resistant E. coli Using Novel Nanoprobe-Combined Fluorescence In Situ Hybridization

1
Environmental Disease Research Center, Korea Research Institute of Bioscience Biotechnology (KRIBB), Daejeon 34141, Korea
2
Department of Nanobiotechnology, KRIBB School of Biotechnology, UST, Daejeon 34113, Korea
3
Infectious Disease Research Center, KRIBB, Daejeon 34141, Korea
4
Bionanotechnology Research Center, KRIBB, Daejeon 34141, Korea
5
Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, UST, Daejeon 34113, Korea
*
Authors to whom correspondence should be addressed.
Nanomaterials 2019, 9(5), 750; https://doi.org/10.3390/nano9050750
Received: 15 April 2019 / Revised: 4 May 2019 / Accepted: 12 May 2019 / Published: 16 May 2019
(This article belongs to the Special Issue Nanoscience and Health: Tiny Technology Raises Big Questions)
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Abstract

Antibiotic-resistant bacteria present a global threat because the infections they cause are difficult to treat. Therefore, it is highly important to develop advanced methods for the identification of antibiotic resistance gene in the virulent bacteria. Here, we report the development of novel nanoprobes for fluorescence in situ hybridization (FISH) and the application of the nanoprobe to the detection of ampicillin-resistant Escherichia coli. The nanoprobe for FISH was synthesized by the modified sol–gel chemistry and the synthesized nanoprobe provided strong fluorescent signals and pH stability even under natural light condition. For the double-identification of bacteria species and ampicillin-resistance with a single probe in situ, the nanoprobes were conjugated to the two kinds of biotinylated probe DNAs; one for E. coli-species specific gene and the other for a drug-resistant gene. By using the nanoprobe-DNA conjugants, we successfully detected the ampicillin-resistant E. coli through the FISH technique. This result suggests the new insight into light stable FISH application of the nanoprobe for a pathogenic antibiotic-resistance bacterium. View Full-Text
Keywords: antibiotic-resistance; ampicillin; bacteria; fluorescence in situ hybridization; nanoprobe antibiotic-resistance; ampicillin; bacteria; fluorescence in situ hybridization; nanoprobe
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Lee, W.S.; Lee, S.; Kang, T.; Ryu, C.-M.; Jeong, J. Detection of Ampicillin-Resistant E. coli Using Novel Nanoprobe-Combined Fluorescence In Situ Hybridization. Nanomaterials 2019, 9, 750.

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