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Nanomaterials 2018, 8(10), 846; https://doi.org/10.3390/nano8100846

Physicochemical Characterization of FRET-Labelled Chitosan Nanocapsules and Model Degradation Studies

1
Institute of Plant Biology and Biotechnology (IBBP), Westfälische Wilhelms-Universität Münster, Schlossplatz 8, 48143 Münster, Germany
2
Department of Dermatology and Venerology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
3
School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK
*
Author to whom correspondence should be addressed.
Received: 17 September 2018 / Revised: 3 October 2018 / Accepted: 16 October 2018 / Published: 17 October 2018
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Abstract

Sub-micron o/w emulsions coated with chitosan have been used for drug delivery, quorum sensing inhibition, and vaccine development. To study interactions with biological systems, nanocapsules have been fluorescently labelled in previous works, but it is often difficult to distinguish the released label from intact nanocapsules. In this study, we present advanced-labelling strategies based on Förster Resonance Energy Transfer (FRET) measurements for chitosan-coated nanocapsules and investigate their dissolution and degradation. We used FRET measurements of nanocapsules loaded with equimolar concentrations of two fluorescent dyes in their oily core and correlated them with dynamic light scattering (DLS) count rate measurement and absorbance measurements during their disintegration by dissolution. Using count rate measurements, we also investigated the enzymatic degradation of nanocapsules using pancreatin and how protein corona formation influences their degradation. Of note, nanocapsules dissolved in ethanol, while FRET decreased simultaneously with count rate, and absorbance was caused by nanocapsule turbidity, indicating increased distance between dye molecules after their release. Nanocapsules were degradable by pancreatin in a dose-dependent manner, and showed a delayed enzymatic degradation after protein corona formation. We present here novel labelling strategies for nanocapsules that allow us to judge their status and an in vitro method to study nanocapsule degradation and the influence of surface characteristics. View Full-Text
Keywords: chitosan; nanocapsules; degradation; FRET; protein corona; bioimaging chitosan; nanocapsules; degradation; FRET; protein corona; bioimaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Hoffmann, S.; Gorzelanny, C.; Moerschbacher, B.; Goycoolea, F.M. Physicochemical Characterization of FRET-Labelled Chitosan Nanocapsules and Model Degradation Studies. Nanomaterials 2018, 8, 846.

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