Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures
2.2. Bulk Heating Protocol
2.3. Prussian Blue Nanoparticle Preparation
2.4. PBNP-PVA Layer Preparation
2.5. Irradiation Protocol by Heating Nanoparticle Layers
2.6. Morphological Analysis
2.7. Electrophysiological Analysis
2.8. Lactate-Dehydrogenase (LDH) Assay
2.9. Statistical Analysis
3. Results
3.1. Effects of Bulk Heating: Morphological and Functional Characterization
3.2. Smart Petri Dish Characterization
3.3. Effects of Thermal Increase by PBNP Irradiation
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bulk Heating (Days 7–8) | |||
---|---|---|---|
37 °C | S.E. | 41.5 °C | S.E. |
4.98 × 10−8 | 1.28 × 10−8 | 4.86 × 10−8 | 1.16 × 10−8 |
Thermal Stimulation (Days 7–8) | |||
---|---|---|---|
CTRL | S.E. | IR | S.E. |
9 × 10−8 | 1.2 × 10−8 | 15 × 10−8 | 2.3 × 10−8 |
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Blasa, S.; Borzenkov, M.; Pastori, V.; Doveri, L.; Pallavicini, P.; Chirico, G.; Lecchi, M.; Collini, M. Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation. Nanomaterials 2022, 12, 2304. https://doi.org/10.3390/nano12132304
Blasa S, Borzenkov M, Pastori V, Doveri L, Pallavicini P, Chirico G, Lecchi M, Collini M. Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation. Nanomaterials. 2022; 12(13):2304. https://doi.org/10.3390/nano12132304
Chicago/Turabian StyleBlasa, Stefania, Mykola Borzenkov, Valentina Pastori, Lavinia Doveri, Piersandro Pallavicini, Giuseppe Chirico, Marzia Lecchi, and Maddalena Collini. 2022. "Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation" Nanomaterials 12, no. 13: 2304. https://doi.org/10.3390/nano12132304