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Article

Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation

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Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
2
Department of Medicine and Surgery, Nanomedicine Center, University of Milano-Bicocca, Via Alfred Nobel, 20854 Vedano al Lambro, Italy
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Department of Chemistry, University of Pavia, Via Torquato Taramelli 12, 27100 Pavia, Italy
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Department of Physics “G. Occhialini”, University of Milano-Bicocca, Piazza della Scienza 3, 20126 Milan, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Angelina Angelova
Nanomaterials 2022, 12(13), 2304; https://doi.org/10.3390/nano12132304
Received: 6 May 2022 / Revised: 27 June 2022 / Accepted: 28 June 2022 / Published: 4 July 2022
(This article belongs to the Section Biology and Medicines)
Heating has recently been applied as an alternative to electrical stimulation to modulate excitability and to induce neuritogenesis and the expression of neuronal markers; however, a long-term functional differentiation has not been described so far. Here, we present the results obtained by a new approach for scalable thermal stimulation on the behavior of a model of dorsal root ganglion neurons, the F-11 cell line. Initially, we performed experiments of bulk stimulation in an incubator for different time intervals and temperatures, and significant differences in neurite elongation and in electrophysiological properties were observed in cultures exposed at 41.5 °C for 30 min. Thus, we exposed the cultures to the same temperature increase using a near-infrared laser to irradiate a disc of Prussian blue nanoparticles and poly-vinyl alcohol that we had adhered to the outer surface of the petri dish. In irradiated cells, neurites were significantly longer, and the electrophysiological properties (action potential firing frequency and spontaneous activity) were significantly increased compared to the control. These results show for the first time that a targeted thermal stimulation could induce morphological and functional neuronal differentiation and support the future application of this method as a strategy to modify neuronal behavior in vivo. View Full-Text
Keywords: nanoparticles; thermal stimulation; neuronal differentiation; neurite outgrowth; electrical activity; electrophysiology nanoparticles; thermal stimulation; neuronal differentiation; neurite outgrowth; electrical activity; electrophysiology
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MDPI and ACS Style

Blasa, S.; Borzenkov, M.; Pastori, V.; Doveri, L.; Pallavicini, P.; Chirico, G.; Lecchi, M.; Collini, M. Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation. Nanomaterials 2022, 12, 2304. https://doi.org/10.3390/nano12132304

AMA Style

Blasa S, Borzenkov M, Pastori V, Doveri L, Pallavicini P, Chirico G, Lecchi M, Collini M. Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation. Nanomaterials. 2022; 12(13):2304. https://doi.org/10.3390/nano12132304

Chicago/Turabian Style

Blasa, Stefania, Mykola Borzenkov, Valentina Pastori, Lavinia Doveri, Piersandro Pallavicini, Giuseppe Chirico, Marzia Lecchi, and Maddalena Collini. 2022. "Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation" Nanomaterials 12, no. 13: 2304. https://doi.org/10.3390/nano12132304

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