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Article

Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires

1
NanoLund, Lund University, Box 118, 22100 Lund, Sweden
2
Solid State Physics, Lund University, Box 118, 22100 Lund, Sweden
3
AlignedBio AB, Medicon Village, Scheeletorget 1, 223 63 Lund, Sweden
4
CREATE Health Translational Cancer Center, Department of Immunotechnology, Lund University, Medicon Village Bldg 406, 223 63 Lund, Sweden
5
Department of Physics, Chalmers University of Technology, 41296 Gothenburg, Sweden
*
Author to whom correspondence should be addressed.
Nanomaterials 2021, 11(1), 227; https://doi.org/10.3390/nano11010227
Received: 23 December 2020 / Revised: 13 January 2021 / Accepted: 14 January 2021 / Published: 16 January 2021
Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO2-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays. View Full-Text
Keywords: antibody microarray; nanowire sensors; biomarker discovery antibody microarray; nanowire sensors; biomarker discovery
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MDPI and ACS Style

Verardo, D.; Liljedahl, L.; Richter, C.; Agnarsson, B.; Axelsson, U.; Prinz, C.N.; Höök, F.; Borrebaeck, C.A.K.; Linke, H. Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires. Nanomaterials 2021, 11, 227. https://doi.org/10.3390/nano11010227

AMA Style

Verardo D, Liljedahl L, Richter C, Agnarsson B, Axelsson U, Prinz CN, Höök F, Borrebaeck CAK, Linke H. Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires. Nanomaterials. 2021; 11(1):227. https://doi.org/10.3390/nano11010227

Chicago/Turabian Style

Verardo, Damiano, Leena Liljedahl, Corinna Richter, Björn Agnarsson, Ulrika Axelsson, Christelle N. Prinz, Fredrik Höök, Carl A. K. Borrebaeck, and Heiner Linke. 2021. "Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires" Nanomaterials 11, no. 1: 227. https://doi.org/10.3390/nano11010227

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