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Open AccessArticle

Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO2 Nanomaterials Following Uptake and Sub-Cellular Localization

1
Institute of Cardiovascular Science, College of Medical Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
2
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
3
School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK
4
Promethean Particles Ltd., 1-3 Genesis Park, Midland Way, Nottingham NG7 3EF, UK
5
Boston College, Higgins 644A, 140 Commonwealth Ave, Chestnut Hill, MA 02467, USA
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(3), 401; https://doi.org/10.3390/nano10030401
Received: 18 January 2020 / Revised: 20 February 2020 / Accepted: 20 February 2020 / Published: 25 February 2020
Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO2 NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO2 NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO2 NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces. View Full-Text
Keywords: nanosafety; protein corona; bionano-interface; cellular uptake; cellular localization; co-localisation; reflectance imaging nanosafety; protein corona; bionano-interface; cellular uptake; cellular localization; co-localisation; reflectance imaging
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MDPI and ACS Style

Khan, A.O.; Di Maio, A.; Guggenheim, E.J.; Chetwynd, A.J.; Pencross, D.; Tang, S.; Belinga-Desaunay, M.-F.A.; Thomas, S.G.; Rappoport, J.Z.; Lynch, I. Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO2 Nanomaterials Following Uptake and Sub-Cellular Localization. Nanomaterials 2020, 10, 401. https://doi.org/10.3390/nano10030401

AMA Style

Khan AO, Di Maio A, Guggenheim EJ, Chetwynd AJ, Pencross D, Tang S, Belinga-Desaunay M-FA, Thomas SG, Rappoport JZ, Lynch I. Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO2 Nanomaterials Following Uptake and Sub-Cellular Localization. Nanomaterials. 2020; 10(3):401. https://doi.org/10.3390/nano10030401

Chicago/Turabian Style

Khan, Abdullah O.; Di Maio, Alessandro; Guggenheim, Emily J.; Chetwynd, Andrew J.; Pencross, Dan; Tang, Selina; Belinga-Desaunay, Marie-France A.; Thomas, Steven G.; Rappoport, Joshua Z.; Lynch, Iseult. 2020. "Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO2 Nanomaterials Following Uptake and Sub-Cellular Localization" Nanomaterials 10, no. 3: 401. https://doi.org/10.3390/nano10030401

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