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Open AccessArticle

Cultivation of Human Microvascular Endothelial Cells on Topographical Substrates to Mimic the Human Corneal Endothelium

by 1,2,†, 3,† and 1,3,4,*
1
T-Lab, #05-01, The Mechanobiology Institute Singapore, National University of Singapore, 5A Engineering Drive 1, 117411, Singapore
2
Duke-NUS Graduate Medical School Singapore, 8 College Road, 169857, Singapore
3
Department of Bioengineering, National University of Singapore, EA-03-12, 9 Engineering Drive 1, 117576,Singapore
4
Department of Surgery, National University of Singapore, 119228, Singapore
*
Author to whom correspondence should be addressed.
Authors contributed equally to the work.
J. Funct. Biomater. 2013, 4(1), 38-58; https://doi.org/10.3390/jfb4010038
Received: 31 October 2012 / Revised: 30 January 2013 / Accepted: 25 February 2013 / Published: 21 March 2013
(This article belongs to the Special Issue Advances in Ophthalmic Biomaterials)
Human corneal endothelial cells have a limited ability to replicate in vivo and in vitro. Allograft transplantation becomes necessary when an accident or trauma results in excessive cell loss. The reconstruction of the cornea endothelium using autologous cell sources is a promising alternative option for therapeutic or in vitro drug testing applications. The native corneal endothelium rests on the Descemet’s membrane, which has nanotopographies of fibers and pores. The use of synthetic topographies mimics the native environment, and it is hypothesized that this can direct the behavior and growth of human microvascular endothelial cells (HMVECs) to resemble the corneal endothelium. In this study, HMVECs are cultivated on substrates with micron and nano-scaled pillar and well topographies. Closely packed HMVEC monolayers with polygonal cells and well-developed tight junctions were formed on the topographical substrates. Sodium/potassium (Na+/K+) adenine triphosphatase (ATPase) expression was enhanced on the microwells substrate, which also promotes microvilli formation, while more hexagonal-like cells are found on the micropillars samples. The data obtained suggests that the use of optimized surface patterning, in particular, the microtopographies, can induce HMVECs to adopt a more corneal endothelium-like morphology with similar barrier and pump functions. The mechanism involved in cell contact guidance by the specific topographical features will be of interest for future studies. View Full-Text
Keywords: nanotopography; cornea endothelium; cornea regeneration; microvascular endothelial cells; contact guidance nanotopography; cornea endothelium; cornea regeneration; microvascular endothelial cells; contact guidance
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MDPI and ACS Style

Chua, J.S.; Liew, L.X.; Yim, E.K.F. Cultivation of Human Microvascular Endothelial Cells on Topographical Substrates to Mimic the Human Corneal Endothelium. J. Funct. Biomater. 2013, 4, 38-58. https://doi.org/10.3390/jfb4010038

AMA Style

Chua JS, Liew LX, Yim EKF. Cultivation of Human Microvascular Endothelial Cells on Topographical Substrates to Mimic the Human Corneal Endothelium. Journal of Functional Biomaterials. 2013; 4(1):38-58. https://doi.org/10.3390/jfb4010038

Chicago/Turabian Style

Chua, Jie S.; Liew, Li X.; Yim, Evelyn K.F. 2013. "Cultivation of Human Microvascular Endothelial Cells on Topographical Substrates to Mimic the Human Corneal Endothelium" J. Funct. Biomater. 4, no. 1: 38-58. https://doi.org/10.3390/jfb4010038

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