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J. Clin. Med. 2019, 8(4), 504; https://doi.org/10.3390/jcm8040504

Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

1
Department of Surgical Science, Plastic and Reconstructive Surgery Unit, University of Rome Tor Vergata, 00133 Rome, Italy
2
The Oncologic and Reconstructive Surgery Breast Unit, Oncology Department, Careggi University Hospital, 50134 Florence, Italy
3
Department of Oncologic and Reconstructive Breast Surgery, “Breast Unit Integrata di Livorno, Cecina, Piombino, Elba, Azienda USL Toscana nord ovest”, 50132 Livorno, Italy
4
Breast Surgical Oncology Unit, General Hospital, 41125 Modena, Italy
5
San Rossore Breast Unit, 56122 Pisa, Italy
*
Author to whom correspondence should be addressed.
Received: 16 March 2019 / Revised: 31 March 2019 / Accepted: 8 April 2019 / Published: 12 April 2019
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Abstract

The areas in which Stromal Vascular Fraction cells (SVFs) have been used include radiotherapy based tissue damage after mastectomy, breast augmentation, calvarial defects, Crohn’s fistulas, and damaged skeletal muscle. Currently, the authors present their experience using regenerative cell therapy in breast reconstruction. The goal of this study was to evaluate the safety and efficacy of the use of Engineered Fat Graft Enhanced with Adipose-derived Stromal Vascular Fraction cells (EF-e-A) in breast reconstruction. 121 patients that were affected by the outcomes of breast oncoplastic surgery were treated with EF-e-A, comparing the results with the control group (n = 50) treated with not enhanced fat graft (EF-ne-A). The preoperative evaluation included a complete clinical examination, a photographic assessment, biopsy, magnetic resonance (MRI) of the soft tissue, and ultrasound (US). Postoperative follow-up took place at two, seven, 15, 21, 36 weeks, and then annually. In 72.8% (n = 88) of breast reconstruction treated with EF-e-A, we observed a restoration of the breast contour and an increase of 12.8 mm in the three-dimensional volume after 12 weeks, which was only observed in 27.3% (n = 33) of patients in the control group that was treated with EF-ne-A. Transplanted fat tissue reabsorption was analyzed with instrumental MRI and US. Volumetric persistence in the study group was higher (70.8%) than that in the control group (41.4%) (p < 0.0001 vs. control group). The use of EF-e-A was safe and effective in this series of treated cases. View Full-Text
Keywords: Adipose-derived stem cells; ASCs; Breast augmentation fat graft; Engineered fat graft Adipose-derived stem cells; ASCs; Breast augmentation fat graft; Engineered fat graft
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Gentile, P.; Casella, D.; Palma, E.; Calabrese, C. Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction. J. Clin. Med. 2019, 8, 504.

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