Seminal Oxidative Stress and Sperm DNA Fragmentation in Men from Couples with Infertility or Unexplained Recurrent Pregnancy Loss
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Study Subjects
General Inclusion and Exclusion Criteria
2.3. Semen Analysis
2.4. Statistical Analysis
3. Results
3.1. Semen Parameters
Correlation between Semen Parameters
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | RPL | Infertile | Controls | p-Value (RPL-Infertile) | p-Value (RPL-Controls) | p-Value (Infertile-Controls) |
---|---|---|---|---|---|---|
Age (years) | 34.7 (30.1–40.0) | 30.7 (29.8–36.3) | 31.3 (29.2–33.1) | 0.35 | 0.008 * | 0.41 |
Body mass index (kg/m2) | 25.6 (23.7–29.8) | 25.6 (23.1–27.8) | 26.0 (23.6–28.3) | 1.00 | 1.00 | 1.00 |
Exercise (hours/week) | 2.5 (0.0–4.5) | 2.5 (1.0–3.5) | 2.5 (1.0–5.0) | 1.00 | 1.00 | 1.00 |
Alcohol (units/week) | 3.5 (1.0–10.0) | 2.5 (1.0–6.5) | 1.75 (1.0–5.5) | 0.87 | 0.18 | 1.00 |
Smokers (%) | 26.7% | 31.0% | 3.3% | 0.78 | 0.026 * | 0.006 * |
Dietary supplementation (%) | 23.3% | 44.8% | 43.3% | 0.10 | 0.17 | 1.00 |
Age of female partner (years) | 32.2 (28.5–36.1) | 30.1 (29.2–31.4) | 29.7 (27.2–31.4) | 0.17 | 0.004 * | 0.49 |
Parameter | RPL | Infertile | Controls | p-Value (RPL-Infertile) | p-Value (RPL-Controls) | p-Value (Infertile-Controls) |
---|---|---|---|---|---|---|
SDF (%) | 13.58 (11.71–19.37) | 16.33 (12.78–22.25) | 16.65 (14.03–23.67) | {0.44} | {0.020} * | {0.37} |
Normalized sORP (mV/106 sperm mL) | 1.04 (0.64–2.03) | 1.54 (0.78–2.36) | 1.68 (0.70–2.58) | {0.073} | {0.16} | {0.12} |
sORP (mV) | 41.98 ± 17.23 | 44.62 ± 19.72 | 56.22 ± 19.64 | {0.62} | {0.013} * | {0.071} |
Volume (mL) | 3.8 ± 1.5 | 3.7 ± 1.4 | 4.3 ± 1.5 | [0.47] | [0.36] | [0.19] |
Concentration (×106/mL) | 31.42 (23.03–51.10) | 28.17 (17.56–42.17) | 36.76 (21.51–66.00) | [0.48] | [0.60] | [0.18] |
Total sperm count (×106) | 134.25 (78.76–189.74) | 106.34 (53.14–165.20) | 164.14 (91.98–250.85) | [0.28] | [0.59] | [0.054] |
Morphology (%) | 4.4 (2.9–6.9) | 2.9 (1.9–4.9) | 2.9 (1.9–4.9) | [0.037] * | [0.083] | [0.54] |
Total motility (%) | 51.97 (34.32–69.42) | 25.88 (18.09–40.19) | 54.38 (40.45–69.19) | {0.099} | {0.66} | {0.056} |
Progressive motility (%) | 30.17 (15.52–48.88) | 11.18 (5.12–20.56) | 32.54 (24.44–43.95) | {0.12} | {0.83} | {0.073} |
Rapid progressive motility (%) | 6.39 (2.04–10.89) | 1.86 (0.28–3.88) | 4.76 (1.99–13.46) | {0.47} | {0.97} | {0.42} |
Mucus penetration ability (×106) | 7.29 (1.42–15.19) | 1.14 (0.16–4.71) | 5.54 (1.83–20.25) | {0.99} | {0.41} | {0.28} |
Correlation | All | RPL | Infertile | Controls | ||||
---|---|---|---|---|---|---|---|---|
r | p-Value | r | p-Value | r | p-Value | r | p-Value | |
SDF—Normalized sORP | 0.36 | <0.001 * | 0.37 | 0.050 | 0.51 | 0.004 * | 0.13 | 0.50 |
SDF—Volume | −0.02 | 0.87 | −0.06 | 0.77 | 0.22 | 0.25 | 0.24 | 0.21 |
Normalized sORP—Volume | 0.17 | 0.11 | 0.04 | 0.85 | 0.23 | 0.22 | 0.18 | 0.35 |
SDF—Concentration | −0.25 | 0.017 * | −0.23 | 0.22 | −0.45 | 0.012 * | −0.08 | 0.70 |
Normalized sORP—Concentration | −0.86 | <0.001 * | −0.82 | <0.001 * | −0.86 | <0.001 * | −0.92 | <0.001 * |
SDF—Morphology | −0.27 | 0.010 * | −0.22 | 0.24 | −0.29 | 0.12 | −0.28 | 0.13 |
Normalized sORP—Morphology | −0.41 | <0.001 * | −0.07 | 0.73 | −0.60 | <0.001 * | −0.44 | 0.015 * |
SDF—Total motility | −0.44 | <0.001 * | −0.42 | 0.021 * | −0.53 | 0.003 * | −0.47 | 0.009 * |
Normalized sORP—Total motility | −0.26 | 0.016 * | 0.05 | 0.80 | −0.68 | <0.001 * | −0.19 | 0.33 |
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Rasmussen, J.M.K.; Dalgaard, M.I.R.; Alipour, H.; Dardmeh, F.; Christiansen, O.B. Seminal Oxidative Stress and Sperm DNA Fragmentation in Men from Couples with Infertility or Unexplained Recurrent Pregnancy Loss. J. Clin. Med. 2024, 13, 833. https://doi.org/10.3390/jcm13030833
Rasmussen JMK, Dalgaard MIR, Alipour H, Dardmeh F, Christiansen OB. Seminal Oxidative Stress and Sperm DNA Fragmentation in Men from Couples with Infertility or Unexplained Recurrent Pregnancy Loss. Journal of Clinical Medicine. 2024; 13(3):833. https://doi.org/10.3390/jcm13030833
Chicago/Turabian StyleRasmussen, Johanne Mejlholm Kold, Maya Isabella Riise Dalgaard, Hiva Alipour, Fereshteh Dardmeh, and Ole Bjarne Christiansen. 2024. "Seminal Oxidative Stress and Sperm DNA Fragmentation in Men from Couples with Infertility or Unexplained Recurrent Pregnancy Loss" Journal of Clinical Medicine 13, no. 3: 833. https://doi.org/10.3390/jcm13030833