Impact of Single Hemodialysis Treatment on immune Cell Subpopulations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients Characteristics and Study Design
2.2. Hemodialysis Technique
2.3. Isolation of Peripheral Blood Mononucleate Cells
2.4. Flow Cytometry Analysis
2.5. Cell Proliferation Analysis
2.6. Annexin V with 7-Aminoactinomycin D (7-AAD) Assay
2.7. Statistical Analysis
3. Results
3.1. Demographic and Dialysis-Related Characteristics
3.2. Effects of HD Session on CD3+T-Cells and Apoptosis
3.3. Dialysis Session Increases the Proliferation Activity of CD4+ T and CD8+ Cells
3.4. Chronic Dialysis Treatment Increases the Percentage of Th17 and Th1 Cells and Decreases the reg/Th17 Ratio
3.5. Dialysis Session Reduces IFN-ꙋ Production by CD8 T Cells
3.6. Dialysis Does Not Alter B Cells Content but Decreases the NK
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Characteristics | Filtryzer BGU1.6 (N = 12) | Theranova (N = 12) | HFR17 (N = 11) | p Value |
---|---|---|---|---|
Age (years) | 74 (54–88) | 74 (56–86) | 76.5 (55.2–87.3) | 0.989 |
Women, n (%) | 6 (50) | 7 (58.3) | 6 (54.5) | 0.925 |
BMI (Kg/m2) | 22.22 (19.87–28.11) | 27.9 (22.15–28) | 24.39 (23.18–32.5) | 0.74 |
Neutrophils (109/L) | 4.3 (3.5–5.5) | 4.4 (3.6–7.3) | 4.3 (3.4–5.3) | 0.939 |
Lymphocytes (109/L) | 1.2 (0.9–1.6) | 1.2 (0.9–1.5) | 1.18 (0.9–1.4) | 0.945 |
Platelets (109/L) | 170 (105–227) | 168 (99.7–224.5) | 170 (101.5–229) | 0.983 |
Ferritin (ng/mL) | 150 (60.7–272.5) | 149.5 (66–319) | 149.5 (63.2–248) | 0.952 |
Transferrin saturation (mg/mL) | 25.7 (12.8–37.2) | 25.5 (13–37.7) | 25.5 (13.4–38) | 0.976 |
Serum iron (µg/dL) | 41 (27.7–78) | 51 (30–78) | 47 (28.5–76.5) | 0.921 |
Albumin (g/dL) | 3.6 (3.3–3.8) | 3.5 (3.4–4.2) | 3.5 (3.4–3.9) | 0.986 |
NLI | 3.8 (2.8–5) | 3.6 (2.9–5) | 3.7 (2.9–4.87) | 0.983 |
PLI | 134 (100–236.5) | 120.7 (89–233) | 134.3 (97.2–230.7) | 0.970 |
CRP (mg/dL) | 0.35 (0.07–6.1) | 0.6 (0.21–0.8) | 0.15 (0.08–0.2) | 0.942 |
Iron therapy (%) | 33.33 | 58.33 | 36.36 | / |
EPO therapy (%) | 100 | 100 | 100 | / |
Characteristics | Filtryzer BGU1.6 (N = 12) | Theranova (N = 12) | HFR17 (N = 11) | p Value |
---|---|---|---|---|
Time spent on dialysis (months) | 47 (19.5–60) | 44 (17.5–60) | 44.5 (15.5–60) | 0.903 |
Type of dialysis (stHD/HDF) | 9/3 | 12/0 | 0/11 | / |
Vascular access (AVF/CVC) | 6/6 | 5/7 | 8/3 | / |
Blood flow (mL/min) | 300 (270–300) | 300 (275–300) | 300 (280–300) | 0.967 |
Dialysis duration (min) | 240 (240–240) | 240 (240–240) | 240 (240–240) | 0.809 |
Session per week | 3 | 3 | 3 | / |
V men (L) | 17.8 (17–21.3) | 17.03 (15.1–18.9) | 18.6 (17.3–20.1) | 0.922 |
V Women (L) | 13.3 (12.3–14.3) | 14.34 (13.3–16.1) | 17.8 (15.3–23.9) | 0.934 |
Phosphorous (mg/dL) | 4.65 (3.85–6.38) | 5.1 (3.9–6.2) | 5.5 (4.35–6.08) | 0.810 |
PTH | 305 (145.8–546.8) | 300 (116–473) | 314 (190–340) | 0.72 |
Calcium (mg/dL) | 8.9 (8.08–9.5) | 8.3 (7.9–8.8) | 8.8 (8.73–9.22) | 0.881 |
Delta on dry weight | 3.1 (2.07–4.05) | 2.2 (2–2.7) | 3.1 (2–3.5) | 0.69 |
Characteristics | Filtryzer BGU1.6 (N = 12) | Theranova (N = 12) | HFR17 (N = 11) | p Value |
---|---|---|---|---|
Urea (mg/dL) | 141 (83–152.5) | 122 (83–165) | 112.5 (98.5–137.8) | 0.673 |
B2M | 34.5 (27.8–33.4) | 29.9 (27.85–33.4) | 31.2 (25.3–42.6) | 0.864 |
Potassium (mEq/L) | 4.9 (4.35–5.15) | 4.5 (4.3–5.7) | 5.95 (5.28–6.08) | 0.791 |
Kappa chains (mg/L) | 144.6 (118.2–224.8) | 165.3 (110.1–211.6) | 215 (123–301.4) | 0.513 |
Lamda chains (mg/L) | 127.2 (62.75–164.5) | 133.6 (94.8–138.3) | 121.3 (105.7–142) | 0.786 |
Global kt/V | 1.58 (14.83–1.68) | 1.64 (1.52–1.67) | 1.66 (1.51–1.74) | 0.834 |
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Donadei, C.; Angeletti, A.; Pizzuti, V.; Zappulo, F.; Conte, D.; Cappuccilli, M.; Chiocchini, A.L.; Scrivo, A.; Apuzzo, D.; Mariggiò, M.A.; et al. Impact of Single Hemodialysis Treatment on immune Cell Subpopulations. J. Clin. Med. 2023, 12, 3107. https://doi.org/10.3390/jcm12093107
Donadei C, Angeletti A, Pizzuti V, Zappulo F, Conte D, Cappuccilli M, Chiocchini AL, Scrivo A, Apuzzo D, Mariggiò MA, et al. Impact of Single Hemodialysis Treatment on immune Cell Subpopulations. Journal of Clinical Medicine. 2023; 12(9):3107. https://doi.org/10.3390/jcm12093107
Chicago/Turabian StyleDonadei, Chiara, Andrea Angeletti, Valeria Pizzuti, Fulvia Zappulo, Diletta Conte, Maria Cappuccilli, Anna Laura Chiocchini, Anna Scrivo, Delia Apuzzo, Maria Addolorata Mariggiò, and et al. 2023. "Impact of Single Hemodialysis Treatment on immune Cell Subpopulations" Journal of Clinical Medicine 12, no. 9: 3107. https://doi.org/10.3390/jcm12093107