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Article

The Predominant Role of Arrestin3 in General GPCR Desensitization in Platelets

Laboratory of Veterinary Pathology and Platelet Signaling, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea
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Academic Editor: Alessandro Cannavo
J. Clin. Med. 2021, 10(20), 4743; https://doi.org/10.3390/jcm10204743
Received: 18 August 2021 / Revised: 8 October 2021 / Accepted: 13 October 2021 / Published: 15 October 2021
Arrestins in concert with GPCR kinases (GRKs) function in G protein-coupled receptor (GPCR) desensitization in various cells. Therefore, we characterized the functional differences of arrestin3 versus arrestin2 in the regulation of GPCR signaling and its desensitization in platelets using mice lacking arrestin3 and arrestin2. In contrast to arrestin2, platelet aggregation and dense granule secretion induced by 2-MeSADP, U46619, thrombin, and AYPGKF were significantly potentiated in arrestin3-deficient platelets compared to wild-type (WT) platelets, while non-GPCR agonist CRP-induced platelet aggregation and secretion were not affected. Surprisingly, in contrast to GRK6, platelet aggregation induced by the co-stimulation of serotonin and epinephrine was significantly potentiated in arrestin3-deficient platelets, suggesting the central role of arrestin3 in general GPCR desensitization in platelets. In addition, the second challenge of ADP and AYPGKF restored platelet aggregation in arrestin3-deficient platelets but failed to do so in WT and arrestin2-deficient platelets, confirming that arrestin3 contributes to GPCR desensitization. Furthermore, ADP- and AYPGKF-induced Akt and ERK phosphorylation were significantly increased in arrestin3-deficient platelets. Finally, we found that arrestin3 is critical for thrombus formation in vivo. In conclusion, arrestin3, not arrestin2, plays a central role in the regulation of platelet functional responses and thrombus formation through general GPCR desensitization in platelets. View Full-Text
Keywords: arrestin2; arrestin3; GPCR; desensitization; platelets arrestin2; arrestin3; GPCR; desensitization; platelets
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MDPI and ACS Style

Chaudhary, P.K.; Kim, S.; Kim, S. The Predominant Role of Arrestin3 in General GPCR Desensitization in Platelets. J. Clin. Med. 2021, 10, 4743. https://doi.org/10.3390/jcm10204743

AMA Style

Chaudhary PK, Kim S, Kim S. The Predominant Role of Arrestin3 in General GPCR Desensitization in Platelets. Journal of Clinical Medicine. 2021; 10(20):4743. https://doi.org/10.3390/jcm10204743

Chicago/Turabian Style

Chaudhary, Preeti K., Sanggu Kim, and Soochong Kim. 2021. "The Predominant Role of Arrestin3 in General GPCR Desensitization in Platelets" Journal of Clinical Medicine 10, no. 20: 4743. https://doi.org/10.3390/jcm10204743

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