Next Article in Journal
HCV Core/NS3 Protein Immunization with “N-Terminal Heat Shock gp96 Protein (rNT (gp96))” Induced Strong and Sustained Th1-Type Cytokines in Immunized Mice
Next Article in Special Issue
Chimeric VLPs Based on HIV-1 Gag and a Fusion Rabies Glycoprotein Induce Specific Antibodies against Rabies and Foot-and-Mouth Disease Virus
Previous Article in Journal
Heightened Willingness toward Pneumococcal Vaccination in the Elderly Population in Shenzhen, China: A Cross-Sectional Study during the COVID-19 Pandemic
Previous Article in Special Issue
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration
Open AccessArticle

Dry Formulation of Virus-Like Particles in Electrospun Nanofibers

1
Department of Microbiology & Immunology, School of Biomedical Sciences, University of Otago, P.O. Box 56, 720 Cumberland St, Dunedin 9054, New Zealand
2
Department of Pathology, Dunedin School of Medicine, University of Otago, P.O. Box 56, 720 Cumberland St, Dunedin 9054, New Zealand
3
Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, NSW 2006, Australia
4
School of Pharmacy, University of Otago, P.O. Box 56, 720 Cumberland St, Dunedin 9054, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editors: Esther Blanco and Juan Bárcena
Vaccines 2021, 9(3), 213; https://doi.org/10.3390/vaccines9030213
Received: 22 January 2021 / Revised: 25 February 2021 / Accepted: 25 February 2021 / Published: 3 March 2021
(This article belongs to the Special Issue Development of Vaccines Based on Virus-Like Particles)
Biologics can be combined with liquid polymer materials and electrospun to produce a dry nanofibrous scaffold. Unlike spray-drying and freeze-drying, electrospinning minimizes the physiological stress on sensitive materials, and nanofiber mat properties such as hydrophobicity, solubility, and melting temperature can be tuned based on the polymer composition. In this study, we explored the dry formulation of a virus-like particle (VLP) vaccine by electrospinning VLP derived from rabbit hemorrhagic disease virus modified to carry the MHC-I gp100 tumor-associated antigen epitope. VLP were added to a polyvinylpyrrolidone (PVP) solution (15% w/v) followed by electrospinning at 24 kV. Formation of a nanofibrous mat was confirmed by scanning electron microscopy, and the presence of VLP was confirmed by transmission electron microscopy and Western blot. VLP from the nanofibers induced T-cell activation and interferon- (IFN-) γ production in vitro. To confirm in vivo cytotoxicity, Pmel mice treated by injection with gp100 VLP from nanofibers induced a gp100 specific immune response, lysing approximately 65% of gp100-pulsed target cells, comparable to mice vaccinated with gp100 VLP in PBS. VLP from nanofibers also induced an antibody response. This work shows that electrospinning can be used to dry-formulate VLP, preserving both humoral and cell-mediated immunity. View Full-Text
Keywords: electrospinning; nanofiber; dry-formulation; virus-like particle; vaccine electrospinning; nanofiber; dry-formulation; virus-like particle; vaccine
Show Figures

Graphical abstract

MDPI and ACS Style

Dowlath, S.; Campbell, K.; Al-Barwani, F.; Young, V.L.; Young, S.L.; Walker, G.F.; Ward, V.K. Dry Formulation of Virus-Like Particles in Electrospun Nanofibers. Vaccines 2021, 9, 213. https://doi.org/10.3390/vaccines9030213

AMA Style

Dowlath S, Campbell K, Al-Barwani F, Young VL, Young SL, Walker GF, Ward VK. Dry Formulation of Virus-Like Particles in Electrospun Nanofibers. Vaccines. 2021; 9(3):213. https://doi.org/10.3390/vaccines9030213

Chicago/Turabian Style

Dowlath, Sasheen; Campbell, Katrin; Al-Barwani, Farah; Young, Vivienne L.; Young, Sarah L.; Walker, Greg F.; Ward, Vernon K. 2021. "Dry Formulation of Virus-Like Particles in Electrospun Nanofibers" Vaccines 9, no. 3: 213. https://doi.org/10.3390/vaccines9030213

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop