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Open AccessArticle

Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid

Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy
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Author to whom correspondence should be addressed.
The authors consider that the first two authors should be regarded as joint first authors.
Current affiliation: GlaxoSmithKline (GSK), 53100 Siena, Italy.
Antioxidants 2020, 9(10), 927; https://doi.org/10.3390/antiox9100927
Received: 24 August 2020 / Revised: 15 September 2020 / Accepted: 24 September 2020 / Published: 28 September 2020
(This article belongs to the Special Issue Dietary Antioxidants for Modulating the Aging Processes)
The oxidative stress, characterized by the imbalance between pro-oxidants and antioxidants molecules, seems to be involved in the pathogenesis of female subfertility. In particular, the presence of different markers of oxidative stress has been reported in human follicular fluid (FF) surrounding oocytes. Based on its distinctive composition and on the close proximity to the oocyte, FF creates a unique microenvironment having a direct impact on oocyte quality, implantation, and early embryo development. An imbalance in reactive oxygen species (ROS) production in ovarian follicular fluid may have a negative effect on these processes and, as a consequence, on female fertility. Therefore, the aim of this study was to evaluate the redox state of the FF through various methodological approaches. By means of 2D-electrophoresis we demonstrated that the main structural changes occurring in the proteins of the follicular fluid of normovulatory women were correlated to the age of the patients and to the antioxidant defenses present in the FF. Measurement of these parameters could have clinical relevance, since the assessment of the oxidative stress rate may be helpful in evaluating in vitro fertilization potential. View Full-Text
Keywords: human follicular fluid; aging; oxidative stress; reactive oxygen species (ROS) human follicular fluid; aging; oxidative stress; reactive oxygen species (ROS)
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Luddi, A.; Governini, L.; Capaldo, A.; Campanella, G.; De Leo, V.; Piomboni, P.; Morgante, G. Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid. Antioxidants 2020, 9, 927.

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