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Open AccessArticle

Anti-Microbiological, Anti-Hyperglycemic and Anti-Obesity Potency of Natural Antioxidants in Fruit Fractions of Saskatoon Berry

1
Department of Fermentation and Cereals Technology, Wrocław University of Environmental and Life Science, 37, Chełmońskiego Street, 51-630 Wroclaw, Poland
2
Department of Food Technology and Human Nutrition, Faculty of Biology and Agriculture, University of Rzeszów, Zelwerowicza 4 St., 35-601 Rzeszów, Poland
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Departament of Pomology, West Pomeranian University of Technology, J. Słowackiego 17 Str, 71-434 Szczecin, Poland
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Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Science, 37, Chełmońskiego Street, 51-630 Wroclaw, Poland
5
Research Institute of Horticulture, Department of Horticultural Crop Breeding, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(9), 397; https://doi.org/10.3390/antiox8090397
Received: 29 August 2019 / Revised: 10 September 2019 / Accepted: 10 September 2019 / Published: 13 September 2019
(This article belongs to the Section Natural and Synthetic Antioxidants)
The aim of the present work was to evaluate for content of phytochemicals (monophosphate nucleotides, free amino acids, polyphenols), and for anti-microbiological, anti-diabetic (ability to inhibit pancreatic lipase, α-glucosidase, and α-amylase), and antioxidant activities in seven selected fruit and fruit fractions of Amelanchier alnifolia. Most of the fruit and fruit fractions analyzed in this study have not been examined in this respect until now. The content of monophosphate nucleotides and free amino acids were tested by ultra-performance liquid chromatography coupled with photodiode array detector and electrospray ionization-mass spectrometry (UPLC-PDA-ESI-MS). The distribution of the examined compounds and biological activity differed significantly depending on the tested fruit and parts of the fruit. Cultivars “Smoky” and “Thiessen” had a high content of essential free amino acids, monophosphate nucleotides, and the highest antioxidant activity. They were also accountable for the high ability to inhibit Enterococcus hirae (anti-bacterial activity), of activity toward α-amylase, α-glucosidase, and pancreatic lipase. Moreover, the fruit peel was abundant in polyphenolic compounds and showed the highest antioxidative activity, which were strongly correlated with each other. In addition, the peel was characterized by a high concentration of monophosphate nucleotides, free amino acids, and were responsible above all for the strong ability to inhibit pancreatic lipase enzymes contributing to the development of obesity. The seeds were rich in uridine 5’-monophosphate, and total essential and non-essential free amino acids, whose contents correlated with the inhibitory activity toward α-amylase and α-glucosidase. The fruit flesh showed a high content of total free amino acids (hydroxy-L-proline, O-phosphoethanolamine, L-citruline). There was a positive correlation between antioxidant capacity and the content of polyphenolic compounds, nucleotide, and ability to inhibit pancreatic lipase, and between anti-hyperglycemic and free amino acids in fruits and fruit fractions. Therefore, the tested fruit of A. alnifolia and their fractions could be essential ingredients of new functional products and/or probiotic food. View Full-Text
Keywords: Amelanchier alnifolia; biological activity; polyphenol compounds; free amino acids; anti-diabetic; in vitro; PCA Amelanchier alnifolia; biological activity; polyphenol compounds; free amino acids; anti-diabetic; in vitro; PCA
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Lachowicz, S.; Wiśniewski, R.; Ochmian, I.; Drzymała, K.; Pluta, S. Anti-Microbiological, Anti-Hyperglycemic and Anti-Obesity Potency of Natural Antioxidants in Fruit Fractions of Saskatoon Berry. Antioxidants 2019, 8, 397.

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