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Open AccessArticle

Influence of Sampling Conditions, Salivary Flow, and Total Protein Content in Uric Acid Measurements in Saliva

1
Neuromove Research Group, Faculty of Sport, Catholic University of San Antonio (UCAM), Campus de los Jerónimos, 30107 Murcia, Spain
2
Faculty of Health Science, Universidad Europea de Canarias, la Orotava, 38300 Tenerife, Spain
3
Interdisciplinary Laboratory of Clinical Analysis, University of Murcia, 30100 Murcia, Spain
4
Garcia Cugat Foundation, 08023 Barcelona, Spain
5
Centre for Sport Studies, Rey Juan Carlos University, Fuenlabrada, 28943 Madrid, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2019, 8(9), 389; https://doi.org/10.3390/antiox8090389
Received: 26 July 2019 / Revised: 31 August 2019 / Accepted: 6 September 2019 / Published: 11 September 2019
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
Uric acid (UA) is the most abundant antioxidant compound in saliva and one of the most sensitive biomarkers for detecting changes in the oxidative status of the organism. The aim of this study was to evaluate the effect of: (i) different methods of saliva sampling and (ii) the correction by salivary flow or total protein on UA concentrations in saliva. Paired saliva (collected by two different methods, passive drooling and using Salivette cotton rolls) and serum samples were obtained from 12 healthy men after the performance of two resistance training exercises of different level of effort that can produce different concentrations in UA in saliva. There were no significant differences between values of uric acid in saliva using Salivette and passive drool. Correlations between UA in serum and saliva and increases in UA in saliva after exercise were detected when saliva samples were obtained by passive drool and Salivette and were not corrected by salivary flow or total protein concentration. Therefore for UA measurements in saliva it would not be recommended to normalize the results by salivary flow or protein concentration. This study highlights the importance of choosing an adequate sampling method selection as well as the expression of results when analytes are measured in saliva. View Full-Text
Keywords: squat; salivettes; biomarkers; oxidative stress squat; salivettes; biomarkers; oxidative stress
MDPI and ACS Style

González-Hernández, J.M.; Franco, L.; Colomer-Poveda, D.; Martinez-Subiela, S.; Cugat, R.; Cerón, J.J.; Márquez, G.; Martínez-Aranda, L.M.; Jimenez-Reyes, P.; Tvarijonaviciute, A. Influence of Sampling Conditions, Salivary Flow, and Total Protein Content in Uric Acid Measurements in Saliva. Antioxidants 2019, 8, 389.

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