(Photo)toxicity of Partially Oxidized Docosahexaenoate and Its Effect on the Formation of Lipofuscin in Cultured Human Retinal Pigment Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of 22:6PC Lipid Vesicles (Liposomes)
2.3. Oxidation of 22:6PC
2.4. Monitoring of 22:6PC Oxidation
2.4.1. Extraction of Lipids
2.4.2. UV–Visible Absorption Spectroscopy
2.4.3. HPLC
2.5. Preparation of Lipid Samples Containing Lipophilic Antioxidants
2.6. Cell Culture
2.7. Exposure of Cells to Partly Oxidized 22:6PC and/or Light
2.8. Cytotoxicity Assays
2.9. Apoptosis Assays
2.10. Isolation of Bovine Photoreceptor Outer Segments (POSs)
2.11. Supplementation of Cells with POSs with and Without Partly Oxidized 22:6PC
2.12. Flow Cytometry Analysis of Cell Fluorescence
2.13. Transmission Electron Microscopy (TEM)
2.14. Statistical Analysis
3. Results
3.1. Partial Oxidation of 22:6PC
3.2. Effects of Partly Oxidized 22:6PC on RPE Cell Viability
3.2.1. Effect of Partly Oxidized 22:6PC on RPE Cell Metabolic Activity
3.2.2. Effects of Oxidized Docosahexaenoate on RPE Plasma Membrane Integrity
3.2.3. Apoptotic Changes: Nuclear Condensation and DNA Cleavage
3.3. Effects of Antioxidants on the Toxicity of Partly Oxidized 22:6PC
3.4. Effect of Supplementation of Cells with POSs Enriched in Partly Oxidized 22:6PC on Metabolic Activity and Formation of Lipofuscin-like Granules
3.4.1. Effect of Supplementation of Cells with POSs Enriched in Partly Oxidized 22:6PC on Metabolic Activity
3.4.2. Effect of Supplementation of Cells with POSs Enriched in Partly Oxidized 22:6PC on the Formation of Lipofuscin-like Granules
4. Discussion
4.1. (Photo)toxicity of Partly Oxidized 22:6PC
4.2. Effects of Antioxidants on (Photo)toxicity of Partly Oxidized 22:6PC
4.3. Effects of Partly Oxidized 22:6PC on the Formation of Lipofuscin-like Deposits from POSs
4.4. Limitations of the Study
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bakker, L.M.; Boulton, M.E.; Różanowska, M.B. (Photo)toxicity of Partially Oxidized Docosahexaenoate and Its Effect on the Formation of Lipofuscin in Cultured Human Retinal Pigment Epithelial Cells. Antioxidants 2024, 13, 1428. https://doi.org/10.3390/antiox13111428
Bakker LM, Boulton ME, Różanowska MB. (Photo)toxicity of Partially Oxidized Docosahexaenoate and Its Effect on the Formation of Lipofuscin in Cultured Human Retinal Pigment Epithelial Cells. Antioxidants. 2024; 13(11):1428. https://doi.org/10.3390/antiox13111428
Chicago/Turabian StyleBakker, Linda M., Michael E. Boulton, and Małgorzata B. Różanowska. 2024. "(Photo)toxicity of Partially Oxidized Docosahexaenoate and Its Effect on the Formation of Lipofuscin in Cultured Human Retinal Pigment Epithelial Cells" Antioxidants 13, no. 11: 1428. https://doi.org/10.3390/antiox13111428
APA StyleBakker, L. M., Boulton, M. E., & Różanowska, M. B. (2024). (Photo)toxicity of Partially Oxidized Docosahexaenoate and Its Effect on the Formation of Lipofuscin in Cultured Human Retinal Pigment Epithelial Cells. Antioxidants, 13(11), 1428. https://doi.org/10.3390/antiox13111428