EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Chemicals
2.2. Surgical Procedures and SE Induction
2.3. Western Blot
2.4. Immunohistochemistry and Mitochondrial Morphometry
2.5. Fluoro-Jade B (FJB) Staining
2.6. Data Analysis
3. Results
3.1. EGCG Attenuates SE-Induced CA1 Neuronal Death
3.2. EGCG Diminishes SE-Induced GPx1 Downregulation in CA1 Neurons
3.3. EGCG Ameliorates SE-Induced Aberrant Mitochondrial Elongation in CA1 Neurons
3.4. EGCG Attenuates a Decrease in DRP1 Expression and Its S616 Phosphorylation in CA1 Neurons
3.5. EGCG Enhances ERK1/2 but Not JNK Phosphorylation in CA1 Neurons following SE
3.6. EGCG Ameliorates NF-κB S536 Phosphorylation in CA1 Neurons following SE
3.7. ERK1/2 Inhibition Abrogates the Effect of EGCG on SE-Induced CA1 Neuronal Degeneration
3.8. ERK1/2 Inhibition Abrogates the Effect of EGCG on Mitochondrial Hyperfusion
3.9. U0126 Co-Treatment Inhibits EGCG-Induced DRP1 S616 Phosphorylation without Affecting GPx1 Induction and NF-κB S536 Phosphorylation following SE
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antigen | Hose | Manufacturer (Catalog Number) | Dilution |
---|---|---|---|
DRP1 | Rabbit | Thermo (PA1-16987) | 1:500 (IH) 1:1000 (WB) |
ERK1/2 | Rabbit | Biorbyt (Orb160960) | 1:2000 (WB) |
GPx1 | Sheep | Biosensis (S-072-100) | 1:2000 (IH) 1:10,000 (WB) |
JNK | Rabbit | Proteintech (10023-1-AP) | 1:1000 (WB) |
Mitochondrial marker (Mitochondrial complex IV subunit 1, MTCO1) | Mouse | Abcam (#ab14705) | 1:500 (IH) |
NeuN | Guinea pig | Millipore (#ABN90P) | 1:1000 (IH) |
NF-κB S536 | Rabbit | Abcam (#ab28856) | 1:100 (IH) |
p-DRP1 S616 | Rabbit | Cell Signaling (#4494) | 1:1000 (WB) |
p-ERK1/2 | Rabbit | Millipore (#05-797R) | 1:100 (IH) 1:1000 (WB) |
p-JNK | Rabbit | Millipore (#07-175) | 1:1000 (WB) |
β-actin | Mouse | Sigma (A5316) | 1:5000 (WB) |
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Kim, J.-E.; Kim, T.-H.; Kang, T.-C. EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus. Antioxidants 2023, 12, 966. https://doi.org/10.3390/antiox12040966
Kim J-E, Kim T-H, Kang T-C. EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus. Antioxidants. 2023; 12(4):966. https://doi.org/10.3390/antiox12040966
Chicago/Turabian StyleKim, Ji-Eun, Tae-Hyun Kim, and Tae-Cheon Kang. 2023. "EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus" Antioxidants 12, no. 4: 966. https://doi.org/10.3390/antiox12040966
APA StyleKim, J.-E., Kim, T.-H., & Kang, T.-C. (2023). EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus. Antioxidants, 12(4), 966. https://doi.org/10.3390/antiox12040966