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Article

Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil

1
Centro de Investigaciones Biológicas “Margarita Salas” (CIB), Consejo Superior de Investigaciones Científicas (CSIC), E-28040 Madrid, Spain
2
Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), Consejo Superior de Investigaciones Científicas (CSIC), E-41012 Seville, Spain
3
Johnson Matthey, Cambridge Science Park U260, Cambridge CB4 0FP, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Stanley Omaye
Antioxidants 2022, 11(5), 915; https://doi.org/10.3390/antiox11050915
Received: 31 March 2022 / Revised: 2 May 2022 / Accepted: 3 May 2022 / Published: 6 May 2022
(This article belongs to the Special Issue Dream Peroxygenases)
Vegetable oils are valuable renewable resources for the production of bio-based chemicals and intermediates, including reactive epoxides of industrial interest. Enzymes are an environmentally friendly alternative to chemical catalysis in oxygenation reactions, epoxidation included, with the added advantage of their potential selectivity. The unspecific peroxygenase of Collariella virescens is only available as a recombinant enzyme (rCviUPO), which is produced in Escherichia coli for protein engineering and analytical-scale optimization of plant lipid oxygenation. Engineering the active site of rCviUPO (by substituting one, two, or up to six residues of its access channel by alanines) improved the epoxidation of individual 18-C unsaturated fatty acids and hydrolyzed sunflower oil. The double mutation at the heme channel (F88A/T158A) enhanced epoxidation of polyunsaturated linoleic and α–linolenic acids, with the desired diepoxides representing > 80% of the products (after 99% substrate conversion). More interestingly, process optimization increased (by 100-fold) the hydrolyzate concentration, with up to 85% epoxidation yield, after 1 h of reaction time with the above double variant. Under these conditions, oleic acid monoepoxide and linoleic acid diepoxide are the main products from the sunflower oil hydrolyzate. View Full-Text
Keywords: unspecific peroxygenase (UPO); Collariella virescens; heme access channel; protein engineering; epoxidation; unsaturated fatty acids; sunflower oil; process optimization unspecific peroxygenase (UPO); Collariella virescens; heme access channel; protein engineering; epoxidation; unsaturated fatty acids; sunflower oil; process optimization
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MDPI and ACS Style

Linde, D.; González-Benjumea, A.; Aranda, C.; Carro, J.; Gutiérrez, A.; Martínez, A.T. Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil. Antioxidants 2022, 11, 915. https://doi.org/10.3390/antiox11050915

AMA Style

Linde D, González-Benjumea A, Aranda C, Carro J, Gutiérrez A, Martínez AT. Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil. Antioxidants. 2022; 11(5):915. https://doi.org/10.3390/antiox11050915

Chicago/Turabian Style

Linde, Dolores, Alejandro González-Benjumea, Carmen Aranda, Juan Carro, Ana Gutiérrez, and Angel T. Martínez. 2022. "Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil" Antioxidants 11, no. 5: 915. https://doi.org/10.3390/antiox11050915

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