Sp1-Mediated Prdx6 Upregulation Leads to Clasmatodendrosis by Increasing Its aiPLA2 Activity in the CA1 Astrocytes in Chronic Epilepsy Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Chemicals
2.2. SE Induction and Chronic Epilepsy Model
2.3. Drug Trials, Electrode Implantation, and Quantification of Seizure Activity
2.4. Western Blot
2.5. Immunohistochemistry
2.6. Data Analysis
3. Results
3.1. Prdx6 Is Upregulated in CA1 Astrocytes in the Epileptic Hippocampus
3.2. CDDO-Me and NAC Attenuate Clasmatodendrosis Accompanied by Prdx6 Downregulation
3.3. NAC Inhibits Prdx6 Upregulation Independent of Nrf2
3.4. Sp1 Inhibition Ameliorates Prdx6 Upregulation and Clasmatodendrosis in CA1 Astrocytes
3.5. MJ33 Ameliorates Clasmatodendritic Degeneration in the Epileptic Hippocampus
3.6. Attenuations of Clasmatodendrosis Reduce Seizure Duration, but Not Its Frequency and Severity in Chronic Epilepsy Rats
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, J.-E.; Lee, D.-S.; Kang, T.-C. Sp1-Mediated Prdx6 Upregulation Leads to Clasmatodendrosis by Increasing Its aiPLA2 Activity in the CA1 Astrocytes in Chronic Epilepsy Rats. Antioxidants 2022, 11, 1883. https://doi.org/10.3390/antiox11101883
Kim J-E, Lee D-S, Kang T-C. Sp1-Mediated Prdx6 Upregulation Leads to Clasmatodendrosis by Increasing Its aiPLA2 Activity in the CA1 Astrocytes in Chronic Epilepsy Rats. Antioxidants. 2022; 11(10):1883. https://doi.org/10.3390/antiox11101883
Chicago/Turabian StyleKim, Ji-Eun, Duk-Shin Lee, and Tae-Cheon Kang. 2022. "Sp1-Mediated Prdx6 Upregulation Leads to Clasmatodendrosis by Increasing Its aiPLA2 Activity in the CA1 Astrocytes in Chronic Epilepsy Rats" Antioxidants 11, no. 10: 1883. https://doi.org/10.3390/antiox11101883
APA StyleKim, J.-E., Lee, D.-S., & Kang, T.-C. (2022). Sp1-Mediated Prdx6 Upregulation Leads to Clasmatodendrosis by Increasing Its aiPLA2 Activity in the CA1 Astrocytes in Chronic Epilepsy Rats. Antioxidants, 11(10), 1883. https://doi.org/10.3390/antiox11101883