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Article

Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats

1
Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
2
Institute of Forest Resources Development, Gyeongsangbuk-do, Andong-si, Gyeongsangbuk-do 36605, Korea
3
Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Filomena Nazzaro and Vincenzo De Feo
Antioxidants 2021, 10(3), 439; https://doi.org/10.3390/antiox10030439
Received: 29 January 2021 / Revised: 5 March 2021 / Accepted: 9 March 2021 / Published: 12 March 2021
(This article belongs to the Special Issue Antioxidant and Biological Properties of Plant Extracts)
Pyrus ussuriensis Maxim (Korean pear) has been used for hundreds of years as a traditional herbal medicine for asthma, cough, and atopic dermatitis in Korea and China. Although it was originally shown to possess anti-inflammatory, antioxidant, and antiatopic properties, its gastroprotective effects have not been investigated. In the present study, we evaluated the protective effects of Pyrus ussuriensis Maxim extract (PUE) against ethanol-induced gastritis in rats. The bioactive compound profile of PUE was determined by gas chromatography mass spectroscopy (GC-MS) and high-performance liquid chromatography (HPLC). The gastroprotection of PUE at different doses (250 and 500 mg/kg body weight) prior to ethanol ingestion was evaluated using an in vivo gastritis rat model. Several endpoints were evaluated, including gastric mucosal lesions, cellular degeneration, intracellular damage, and immunohistochemical localization of leucocyte common antigen. The gastric mucosal injury and ulcer score were determined by evaluating the inflamed gastric mucosa and by histological examination. To identify the mechanisms of gastroprotection by PUE, antisecretory action and plasma prostaglandin E2 (PGE2), gastric mucosal cyclic adenosine monophosphate (cAMP), and histamine levels were measured. PUE exhibited significant antioxidant effects with IC50 values of 56.18 and 22.49 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition (%), respectively. In addition, GC/MS and HPLC analyses revealed several bioactive compounds of PUE. Pretreatment with PUE significantly (p < 0.05) decreased the ulcer index by preventing gastric mucosal lesions, erosion, and cellular degeneration. An immunohistochemical analysis revealed that PUE markedly attenuated leucocyte infiltration in a dose-dependent manner. The enhancement of PGE2 levels and attenuation of cAMP levels along with the inhibition of histamine release following PUE pretreatment was associated with the cytoprotective and healing effects of PUE. In contrast, the downregulation of the H+/K+ ATPase pathway as well as muscarinic receptor (M3R) and histamine receptor (H2R) inhibition was also involved in the gastroprotective effects of PUE; however, the expression of cholecystokinin-2 receptors (CCK2R) was unchanged. Finally, no signs of toxicity were observed following PUE treatment. Based on our results, we conclude that PUE represents an effective therapeutic option to reduce the risk of gastritis and warrants further study. View Full-Text
Keywords: Pyrus ussuriensis Maxim; GC-MS; HPLC analysis; antioxidant; IC50; gastritis; ulcer scoring; leucocytes common antigen; prostaglandin E2; H+/K+ ATPase Pyrus ussuriensis Maxim; GC-MS; HPLC analysis; antioxidant; IC50; gastritis; ulcer scoring; leucocytes common antigen; prostaglandin E2; H+/K+ ATPase
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MDPI and ACS Style

Boby, N.; Abbas, M.A.; Lee, E.-B.; Im, Z.-E.; Hsu, W.H.; Park, S.-C. Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats. Antioxidants 2021, 10, 439. https://doi.org/10.3390/antiox10030439

AMA Style

Boby N, Abbas MA, Lee E-B, Im Z-E, Hsu WH, Park S-C. Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats. Antioxidants. 2021; 10(3):439. https://doi.org/10.3390/antiox10030439

Chicago/Turabian Style

Boby, Naila, Muhammad A. Abbas, Eon-Bee Lee, Zi-Eum Im, Walter H. Hsu, and Seung-Chun Park. 2021. "Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats" Antioxidants 10, no. 3: 439. https://doi.org/10.3390/antiox10030439

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